The mechanisms of synaptic tramsmission in the vertebrate retina and its role in visual information processing will be studied by intracellular recording from single neurons in the amphibian retina. The effects of various suspected neurotransmitter substances and specific antagonists on the resting potential, input resistance and light responses of different types of retinal neurons will be examined. The test substances will be applied to the retina in known concentrations by a superfusion system which allows changes to be made in the bathing medium while continuosly recording from a single neuron. Particular questions to be studied are the interaction of excitatory and inhibitory inputs in ganglion cells, the synaptic connections which mediate center-surround antagonism in the inner and outer synaptic layers of the retina, the generation of transient responses in amacrine and ganglion cells, and the types of receptors at which rod cone neurotransmitter substances act on second-order cells in the retina. The immediate aim is to obtain information on the different synpatic inputs to each type of retinal neuron, the transmitter substances which mediate these inputs, and the sources of the inputs. The long-term goal of this research is to develop a more complete model of how different neurons interact to process visual information.

National Institute of Health (NIH)
National Eye Institute (NEI)
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Visual Sciences A Study Section (VISA)
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University of Michigan Ann Arbor
Schools of Medicine
Ann Arbor
United States
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Cook, P B; Lukasiewicz, P D; McReynolds, J S (2000) GABA(C) receptors control adaptive changes in a glycinergic inhibitory pathway in salamander retina. J Neurosci 20:806-12
Cook, P B; Lukasiewicz, P D; McReynolds, J S (1998) Action potentials are required for the lateral transmission of glycinergic transient inhibition in the amphibian retina. J Neurosci 18:2301-8
Ball, A K; McReynolds, J S (1998) Localization of gap junctions and tracer coupling in retinal Muller cells. J Comp Neurol 393:48-57
Cook, P B; McReynolds, J S (1998) Lateral inhibition in the inner retina is important for spatial tuning of ganglion cells. Nat Neurosci 1:714-9
Cook, P B; McReynolds, J S (1998) Modulation of sustained and transient lateral inhibitory mechanisms in the mudpuppy retina during light adaptation. J Neurophysiol 79:197-204
Myhr, K L; McReynolds, J S (1996) Cholinergic modulation of dopamine release and horizontal cell coupling in mudpuppy retina. Vision Res 36:3933-8
Myhr, K L; Dong, C J; McReynolds, J S (1994) Cones contribute to light-evoked, dopamine-mediated uncoupling of horizontal cells in the mudpuppy retina. J Neurophysiol 72:56-62
Dong, C J; McReynolds, J S (1992) Comparison of the effects of flickering and steady light on dopamine release and horizontal cell coupling in the mudpuppy retina. J Neurophysiol 67:364-72
Dong, C J; McReynolds, J S (1992) An intensity-dependent biphasic neuron in mudpuppy retina. Vision Res 32:1405-8
Akopian, A; McReynolds, J; Weiler, R (1991) Short-term potentiation of off-responses in turtle horizontal cells. Brain Res 546:132-8

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