Although the HSV-1 gene(s) that is involved in eye disease is not yet known, we have demonstrated previously that immunization of mice with gK, but not with any other known HSV-1 glycoprotein, significantly exacerbates CS and facial dermatitis following ocular HSV-1 infection of different strains of mice. This exacerbation of the CS occurs independently of the virus strain used for infection or the strain of mouse. As gK is essential to HSV-1 infectivity, we analyzed its contribution to CS using recombinant viruses with two extra copies of gK and found that, similar to gK immunization, mice infected with this recombinant virus have elevated levels of CS. The exacerbation of disease is of particular interest as it would appear to mimic the clinical disease process typical of individuals with a history of HSV-1 recurrences. This provided the basis for our innovative approach to the problem of identifying potential therapeutic targets for prevention of ocular HSV-1 infection and HSV-1- induced CS. Our progress during the past funding period provides novel insights into the potential mechanisms by which gK may regulate primary infections and CS. We have now shown that: (1) The cleaved form of gK binds to signal peptide peptidase (SPP) and increases virus replication (Preliminary Studies) and (2) The 8-mer within the signal sequence of gK following cleavage by SPP up-regulates the CD8+CD25+ responses in the cornea of infected mice leading to exacerbation of CS. Based on these data, we have formulated the central hypothesis that the pathogenic effects of gK during primary infection are mediated by two different, but inter-related, mechanisms in which the cleaved form of gK exerts its activities by binding to SPP leading to higher levels of virus replication, while the 8-mer within the signal sequence of gK following cleavage by SPP up-regulates CD8+CD25+ T cells in vivo leading to greater levels of eye disease. We propose to test this hypothesis through the following Specific Aims:
Aim 1 : Test the hypothesis that binding of the cleaved form of gK to SPP plays a major role in virus replication and thus infectivity in vitro and in vivo.
Aim 2 : Test the hypothesis that the 8-mer (ITAYGLVL) within the signal sequence of gK binds to MHC-I on DCs and mediates up-regulation of CD8+CD25+ T cells, thus leading to exacerbation of CS in ocularly infected mice.

Public Health Relevance

Previously we have reported that HSV-1 gK exacerbate eye disease in infected mice. In Our Preliminary Studies we have shown that gK binds to signal peptide peptidase (SPP). We now plan to demonstrate that blocking this interaction will reduce virus replication in the eye and reduce eye disease in HSV-1 infected mice.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY013615-08A1
Application #
8234590
Study Section
Anterior Eye Disease Study Section (AED)
Program Officer
Mckie, George Ann
Project Start
2001-07-01
Project End
2015-12-31
Budget Start
2012-01-01
Budget End
2012-12-31
Support Year
8
Fiscal Year
2012
Total Cost
$417,500
Indirect Cost
$167,500
Name
Cedars-Sinai Medical Center
Department
Type
DUNS #
075307785
City
Los Angeles
State
CA
Country
United States
Zip Code
90048
Jaggi, Ujjaldeep; Wang, Shaohui; Tormanen, Kati et al. (2018) Role of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein K (gK) Pathogenic CD8+ T Cells in Exacerbation of Eye Disease. Front Immunol 9:2895
Wang, Shaohui; Ljubimov, Alexander V; Jin, Ling et al. (2018) Herpes Simplex Virus 1 Latency and the Kinetics of Reactivation Are Regulated by a Complex Network of Interactions between the Herpesvirus Entry Mediator, Its Ligands (gD, BTLA, LIGHT, and CD160), and the Latency-Associated Transcript. J Virol 92:
Wang, Shaohui; Mott, Kevin R; Wawrowsky, Kolja et al. (2017) Binding of Herpes Simplex Virus 1 UL20 to GODZ (DHHC3) Affects Its Palmitoylation and Is Essential for Infectivity and Proper Targeting and Localization of UL20 and Glycoprotein K. J Virol 91:
Matundan, Harry H; Mott, Kevin R; Allen, Sariah J et al. (2016) Interrelationship of Primary Virus Replication, Level of Latency, and Time to Reactivation in the Trigeminal Ganglia of Latently Infected Mice. J Virol 90:9533-42
Mott, Kevin R; Gate, David; Matundan, Harry H et al. (2016) CD8+ T Cells Play a Bystander Role in Mice Latently Infected with Herpes Simplex Virus 1. J Virol 90:5059-5067
Matundan, Harry H; Mott, Kevin R; Akhtar, Aslam Abbasi et al. (2015) Mutations within the pathogenic region of herpes simplex virus 1 gK signal sequences alter cell surface expression and neurovirulence. J Virol 89:2530-42
Mott, Kevin R; Maazi, Hadi; Allen, Sariah J et al. (2015) Batf3 deficiency is not critical for the generation of CD8?? dendritic cells. Immunobiology 220:518-24
Mott, Kevin R; Allen, Sariah J; Zandian, Mandana et al. (2014) Coregulatory interactions among CD8? dendritic cells, the latency-associated transcript, and programmed death 1 contribute to higher levels of herpes simplex virus 1 latency. J Virol 88:6599-610
Allen, Sariah J; Mott, Kevin R; Ghiasi, Homayon (2014) Inhibitors of signal peptide peptidase (SPP) affect HSV-1 infectivity in vitro and in vivo. Exp Eye Res 123:8-15
Dumitrascu, O M; Mott, K R; Ghiasi, H (2014) A comparative study of experimental mouse models of central nervous system demyelination. Gene Ther 21:599-608

Showing the most recent 10 out of 41 publications