We propose to initiate studies to define mechanisms controlling gene expression in cyanobacteria. Specifically, we propose to isolate and characterize cloned fragments of cyanobacterial DNA encoding for the apoproteins of Synechococcus 7002 allophycocyanin and Synechocystis 6701 phycoerythrin. These cloned DNA fragments, together with cloned fragments already being characterized which carry coding sequences for the apoprotein subunits of Synechcoccus 7002 phycocyanin, will be used as probes to identify and isolate the mRNAs coding for these phycobiliproteins. We will directly assess the role of transcriptional control in the regulation of phycobiliprotein biosynthesis by measuring the intracellular concentrations of biliprotein mRNAs in cells exposed to various environmental stresses including nutrient starvation (for nitrogen, carbon, or phosphate), light intensity (intensity adaptation), and light wavelength (complementary chromatic adaption). We will use cloned cyanobacterial DNA fragments as templates for the development of an in vitro transcription/translation system. Such a system could be useful in the isolation of factors involved in the control of expression of the phycobiliprotein genes. The proposed studies are relevant and of comparative interest in understanding gene regulation processes in both procaryotic and eucaryotic photoautotrophs.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM031625-03
Application #
3279771
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1983-03-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
University Park
State
PA
Country
United States
Zip Code
16802
Shen, Gaozhong; Schluchter, Wendy M; Bryant, Donald A (2008) Biogenesis of phycobiliproteins: I. cpcS-I and cpcU mutants of the cyanobacterium Synechococcus sp. PCC 7002 define a heterodimeric phyococyanobilin lyase specific for beta-phycocyanin and allophycocyanin subunits. J Biol Chem 283:7503-12
Saunee, Nicolle A; Williams, Shervonda R; Bryant, Donald A et al. (2008) Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002. J Biol Chem 283:7513-22
Woodger, Fiona J; Bryant, Donald A; Price, G Dean (2007) Transcriptional regulation of the CO2-concentrating mechanism in a euryhaline, coastal marine cyanobacterium, Synechococcus sp. Strain PCC 7002: role of NdhR/CcmR. J Bacteriol 189:3335-47
Inoue-Sakamoto, Kaori; Gruber, Tanja M; Christensen, Suzanne K et al. (2007) Group 3 sigma factors in the marine cyanobacterium Synechococcus sp. strain PCC 7002 are required for growth at low temperature. J Gen Appl Microbiol 53:89-104
Shen, Gaozhong; Saunee, Nicolle A; Williams, Shervonda R et al. (2006) Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002. J Biol Chem 281:17768-78
Frigaard, Niels-Ulrik; Sakuragi, Yumiko; Bryant, Donald A (2004) Gene inactivation in the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum using in vitro-made DNA constructs and natural transformation. Methods Mol Biol 274:325-40
Wang, Tao; Shen, Gaozhong; Balasubramanian, Ramakrishnan et al. (2004) The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria. J Bacteriol 186:956-67
Yu, Jianping; Shen, Gaozhong; Wang, Tao et al. (2003) Suppressor mutations in the study of photosystem I biogenesis: sll0088 is a previously unidentified gene involved in reaction center accumulation in Synechocystis sp. strain PCC 6803. J Bacteriol 185:3878-87
Gomez-Lojero, Carlos; Perez-Gomez, Bertha; Shen, Gaozhong et al. (2003) Interaction of ferredoxin:NADP+ oxidoreductase with phycobilisomes and phycobilisome substructures of the cyanobacterium Synechococcus sp. strain PCC 7002. Biochemistry 42:13800-11
Huang, Chenhui; Yuan, Xiaolin; Zhao, Jindong et al. (2003) Kinetic analyses of state transitions of the cyanobacterium Synechococcus sp. PCC 7002 and its mutant strains impaired in electron transport. Biochim Biophys Acta 1607:121-30

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