This is a proposal to study the molecular genetics of mating type and sexual development in Schizophyllum commune. This fungus has four multiallelic, mating-type loci, A alpha, A beta, and B beta loci. Representative alleles of each locus will be cloned and sequenced. Regions that are essential for A function will be identified, the number of complementation groups in each locus will be determined and the expression of genes in these loci will be studied. Attempts will be made to identify the gene products and determine their cellular location. Mutations that alter self-nonself recognition of the alleles will be isolated and analyzed. A mutant hunt will be made to identify genes regulated by the A alpha and A beta loci. At present nothing is known about the number of genes at each locus, the gene products, and the differences and similarities between alleles. The proposed studies are expected to answer these questions and they will begin to address the question of how the A loci regulate genes in the A-specific pathways of sexual development. Information obtained in this study of Schizophylum will also contribute to understanding the nature of multiallelic genes and self-recognition in other organisms.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM034023-05
Application #
3284406
Study Section
Genetics Study Section (GEN)
Project Start
1986-07-01
Project End
1994-06-30
Budget Start
1990-07-01
Budget End
1991-06-30
Support Year
5
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of Vermont & St Agric College
Department
Type
Schools of Medicine
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Robertson, C Ian; McMahon Kende, Alexander; Toenjes, Kurt et al. (2002) Evidence for interaction of Schizophyllum commune Y mating-type proteins in vivo. Genetics 160:1461-7
Shen, G P; Chen, Y; Song, D et al. (2001) The Aalpha6 locus: its relation to mating-type regulation of sexual development in Schizophyllum commune. Curr Genet 39:340-5
Asada, Y; Yue, C; Wu, J et al. (1997) Schizophyllum commune A alpha mating-type proteins, Y and Z, form complexes in all combinations in vitro. Genetics 147:117-23
Yue, C; Osier, M; Novotny, C P et al. (1997) The specificity determinant of the Y mating-type proteins of Schizophyllum commune is also essential for Y-Z protein binding. Genetics 145:253-60
Robertson, C I; Bartholomew, K A; Novotny, C P et al. (1996) Deletion of the Schizophyllum commune A alpha locus: the roles of A alpha Y and Z mating-type genes. Genetics 144:1437-44
Wu, J; Ullrich, R C; Novotny, C P (1996) Regions in the Z5 mating gene of Schizophyllum commune involved in Y-Z binding and recognition. Mol Gen Genet 252:739-45
Shen, G P; Park, D C; Ullrich, R C et al. (1996) Cloning and characterization of a Schizophyllum gene with A beta 6 mating-type activity. Curr Genet 29:136-42
Marion, A L; Bartholomew, K A; Wu, J et al. (1996) The A alpha mating-type locus of Schizophyllum commune: structure and function of gene X. Curr Genet 29:143-9
Isaya, G; Sakati, W R; Rollins, R A et al. (1995) Mammalian mitochondrial intermediate peptidase: structure/function analysis of a new homologue from Schizophyllum commune and relationship to thimet oligopeptidases. Genomics 28:450-61
Magae, Y; Novotny, C; Ullrich, R (1995) Interaction of the A alpha Y and Z mating-type homeodomain proteins of Schizophyllum commune detected by the two-hybrid system. Biochem Biophys Res Commun 211:1071-6

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