The goal of this proposal is to understand the mechanism and developmental process in plants. The focus is on the Activator (Ac)/Dissociation (Ds) family of transposons of Zea mays. These elements are members of a large superfamily of elements that transpose via DNA intermediates. We propose a genetic and biochemical approach to dissect the transposition process to identify requisite cis-acting elements and trans-acting factors. These studies exploit both maize developmental genetics and the ability of Ac/Ds clement to function in the heterologous host, Arabidopsis.
Our specific aims are to: i) identify cellular factors required for transposition using saturation mutagenesis including enhancers and suppressor mutations that affect transposition processes; ii) initiate biochemical studies to identify interacting protein factors; iii) investigate a transposon-inactivation process associated with methylation and repeat-induced gene silencing; iv) study how mutations affect the frequency and timing of transposition during development; and v) (attempt to develop in vitro transposition assays to study target site specificity and the biochemical requirements for transposition. These studies should lead us to a better understanding of the mechanism and developmental control of transposition and provide a greater understanding of gene silencing and chromosomal position effects. Studies on transposition will also shed light on the health-related processes of spontaneous mutations, genetic diseases, somatic mutations and genome structure.
|Persson, Bengt; Kallberg, Yvonne; Bray, James E et al. (2009) The SDR (short-chain dehydrogenase/reductase and related enzymes) nomenclature initiative. Chem Biol Interact 178:94-8|
|Acosta, Iván F; Laparra, Hélène; Romero, Sandra P et al. (2009) tasselseed1 is a lipoxygenase affecting jasmonic acid signaling in sex determination of maize. Science 323:262-5|
|Schierwater, Bernd; Eitel, Michael; Jakob, Wolfgang et al. (2009) Concatenated analysis sheds light on early metazoan evolution and fuels a modern ""urmetazoon"" hypothesis. PLoS Biol 7:e20|
|Kim, Jong Cheol; Laparra, Helene; Calderon-Urrea, Alejandro et al. (2007) Cell cycle arrest of stamen initials in maize sex determination. Genetics 177:2547-51|
|Wu, Xiaoqiu; Knapp, Stefan; Stamp, Anna et al. (2007) Biochemical characterization of TASSELSEED 2, an essential plant short-chain dehydrogenase/reductase with broad spectrum activities. FEBS J 274:1172-82|
|Cadavid, Luis F; Powell, Anahid E; Nicotra, Matthew L et al. (2004) An invertebrate histocompatibility complex. Genetics 167:357-65|
|Curie, C; Panaviene, Z; Loulergue, C et al. (2001) Maize yellow stripe1 encodes a membrane protein directly involved in Fe(III) uptake. Nature 409:346-9|
|Schultes, N P; Brutnell, T P; Allen, A et al. (1996) Leaf permease1 gene of maize is required for chloroplast development. Plant Cell 8:463-75|
|Brutnell, T P; Dellaporta, S L (1994) Somatic inactivation and reactivation of Ac associated with changes in cytosine methylation and transposase expression. Genetics 138:213-25|
|Dellaporta, S L; Calderon-Urrea, A (1994) The sex determination process in maize. Science 266:1501-5|
Showing the most recent 10 out of 16 publications