Using a combination of genetics and molecular biology in Drosophila, Hays will study how dynein is targeted to different tissue/cells to perform a variety of functions. He will concentrate on the dynein intermediate and light chains since they have been indicated as having a targeting function. He will generate nulls, analyzing the resulting phenotypes, and will also characterize proteins that interact with the light and intermediate chains. He will carry out studies with dynein mutants to help elucidate the role of dynein in mitosis. These will also involve studies on asymmetric divisions in neuroblasts. In vitro collaborative studies will involve binding of dynein to kinetochores in permeabilized systems. Since dynein binding to cargo appears in many instances to be mediated by dynactin, mutants that modify dynein-dynactin interactions will be characterized. This is a global study of the various motilities that cytoplasmic dynein performs in eukaryotic cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM044757-10A1
Application #
6125406
Study Section
Cell Development and Function Integrated Review Group (CDF)
Program Officer
Deatherage, James F
Project Start
1990-07-01
Project End
2004-02-29
Budget Start
2000-03-01
Budget End
2001-02-28
Support Year
10
Fiscal Year
2000
Total Cost
$254,016
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Genetics
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Tubman, Emily; He, Yungui; Hays, Thomas S et al. (2018) Kinesin-5 mediated chromosome congression in insect spindles. Cell Mol Bioeng 11:25-36
Bhaban, Shreyas; Talukdar, Saurav; Li, Mingang et al. (2018) Single Molecule Studies Enabled by Model-Based Controller Design. IEEE ASME Trans Mechatron 23:1532-1542
Neisch, Amanda L; Neufeld, Thomas P; Hays, Thomas S (2017) A STRIPAK complex mediates axonal transport of autophagosomes and dense core vesicles through PP2A regulation. J Cell Biol 216:441-461
Avery, Adam W; Fealey, Michael E; Wang, Fengbin et al. (2017) Structural basis for high-affinity actin binding revealed by a ?-III-spectrin SCA5 missense mutation. Nat Commun 8:1350
Avery, Adam W; Thomas, David D; Hays, Thomas S (2017) ?-III-spectrin spinocerebellar ataxia type 5 mutation reveals a dominant cytoskeletal mechanism that underlies dendritic arborization. Proc Natl Acad Sci U S A 114:E9376-E9385
Neisch, Amanda L; Avery, Adam W; Machamer, James B et al. (2016) Methods to identify and analyze gene products involved in neuronal intracellular transport using Drosophila. Methods Cell Biol 131:277-309
Mauvezin, Caroline; Neisch, Amanda L; Ayala, Carlos I et al. (2016) Coordination of autophagosome-lysosome fusion and transport by a Klp98A-Rab14 complex in Drosophila. J Cell Sci 129:971-82
Bhaban, Shreyas; Materassi, Donatello; Li, Mingang et al. (2016) Interrogating Emergent Transport Properties for Molecular Motor Ensembles: A Semi-analytical Approach. PLoS Comput Biol 12:e1005152
McIntosh, J Richard; Hays, Thomas (2016) A Brief History of Research on Mitotic Mechanisms. Biology (Basel) 5:
Avery, Adam W; Crain, Jonathan; Thomas, David D et al. (2016) A human ?-III-spectrin spinocerebellar ataxia type 5 mutation causes high-affinity F-actin binding. Sci Rep 6:21375

Showing the most recent 10 out of 25 publications