Abstract

The dsDNA bacteriophages provide an unparalleled resource for investigating the principles of biological assembly at the molecular level. This is a proposal to continue the study of these problems using the E. coli bacteriophages lambda and HK97. Specific interests include assembly of the heads of HK97, with an emphasis on the extensive conformational rearrangement in the capsid protein that happens during DNA packaging, assembly of the phage lambda tail, with an emphasis on the newly discovered chaperone-mediated step in the assembly of the tail length template, and assembly and structure of the lambda long tail fibers. Information gained in this study will be generalizable beyond bacteriophages and will therefore affect our understanding of, among others, assembly processes in animal and plant viruses, which are possible points for anti-viral intervention.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM047795-31
Application #
7269522
Study Section
Prokaryotic Cell and Molecular Biology Study Section (PCMB)
Program Officer
Basavappa, Ravi
Project Start
1977-12-01
Project End
2009-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
31
Fiscal Year
2007
Total Cost
$500,120
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Hua, Jianfei; Huet, Alexis; Lopez, Carlos A et al. (2017) Capsids and Genomes of Jumbo-Sized Bacteriophages Reveal the Evolutionary Reach of the HK97 Fold. MBio 8:
Tso, DanJu; Peebles, Craig L; Maurer, Joshua B et al. (2017) On the catalytic mechanism of bacteriophage HK97 capsid crosslinking. Virology 506:84-91
Hasek, Mary L; Maurer, Joshua B; Hendrix, Roger W et al. (2017) Flexible Connectors between Capsomer Subunits that Regulate Capsid Assembly. J Mol Biol 429:2474-2489
Huet, Alexis; Duda, Robert L; Hendrix, Roger W et al. (2016) Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. J Mol Biol 428:165-181
Casjens, Sherwood R; Hendrix, Roger W (2015) Bacteriophage lambda: Early pioneer and still relevant. Virology 479-480:310-30
Cardone, Giovanni; Duda, Robert L; Cheng, Naiqian et al. (2014) Metastable intermediates as stepping stones on the maturation pathways of viral capsids. MBio 5:e02067
Pope, Welkin H; Jacobs-Sera, Deborah; Russell, Daniel A et al. (2014) Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood. MBio 5:e02145
Xu, Jun; Hendrix, Roger W; Duda, Robert L (2014) Chaperone-protein interactions that mediate assembly of the bacteriophage lambda tail to the correct length. J Mol Biol 426:1004-18
Tso, Dan-ju; Hendrix, Roger W; Duda, Robert L (2014) Transient contacts on the exterior of the HK97 procapsid that are essential for capsid assembly. J Mol Biol 426:2112-29
Oh, Bonnie; Moyer, Crystal L; Hendrix, Roger W et al. (2014) The delta domain of the HK97 major capsid protein is essential for assembly. Virology 456-457:171-8

Showing the most recent 10 out of 36 publications