This continuation proposal is aimed at the design, preparation, and testing of monolithic """"""""molded"""""""" separation media in entirely new shapes and formats that complement current bead-based packed media and provide new and unmatched capabilities for the study, detection, isolation, and separation of biologically active molecules. The new separation media are based on a continuous body of macroporous polymer molded directly into the housing of the separation device rather than made of small beads mechanically packed into the column. The """"""""molded"""""""" media offer many advantages such as ease of preparation and ease of handling, reproducibility, versatility of separation chemistry, unmatched ability to incorporate gradients of porosity and of chemistry, compactness, and simple scale-up. In contrast to the two-phase systems characteristic of the suspension polymerization used to prepare bead-shaped separation media, the molded media are prepared from a single phase allowing the use of many functional or water-soluble monomers that cannot be used for conventional beads. The ease of preparation is another important factor that removes the size fractionation and packing steps which are known to greatly affect the efficiency of packed columns. The preparation of the continuous media is simple, waste-free, and less labor-intensive that the preparation of beads, leading to a low unit cost per device. The versatility of separation chemistry is further enhanced by the ability to blend several chemistries or achieve gradients of composition and/or porosity in a single molded medium. Other advantages of the new molded monoliths include remarkably enhanced mass transfer because all of the liquid phase flows through their large pores that are connected to a network of small pores with a large surface area. This allows extremely fast separations of biopolymers and leads to a dramatic improvement in kinetics for immobilized biocatalysts. Overall, our targets are to significantly enhance the array of analytical, preparative, and diagnostics tools available for handling biological fluids. The new media we develop will contribute to both better performance and new capabilities in areas such as high-performance liquid chromatography, electrochromatography, capillary and free-flow zone electrophoresis, diagnostics, detection, enzyme immobilization, etc.. The unequaled versatility of the molded systems and their unique features make them ideal for a broad range of products from the smallest capillary format to large preparative systems for the separation of drugs, peptides, proteins, and oligo- or polynucleotides.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048364-07
Application #
2857162
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1993-01-01
Project End
1999-12-31
Budget Start
1999-01-01
Budget End
1999-12-31
Support Year
7
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Lv, Yongqin; Lin, Zhixing; Tan, Tianwei et al. (2014) Preparation of reusable bioreactors using reversible immobilization of enzyme on monolithic porous polymer support with attached gold nanoparticles. Biotechnol Bioeng 111:50-8
Lv, Yongqin; Lin, Zhixing; Svec, Frantisek (2012) Hypercrosslinked large surface area porous polymer monoliths for hydrophilic interaction liquid chromatography of small molecules featuring zwitterionic functionalities attached to gold nanoparticles held in layered structure. Anal Chem 84:8457-60
Lv, Yongqin; Lin, Zhixing; Svec, Frantisek (2012) ""Thiol-ene"" click chemistry: a facile and versatile route for the functionalization of porous polymer monoliths. Analyst 137:4114-8
Svec, Frantisek (2012) Quest for organic polymer-based monolithic columns affording enhanced efficiency in high performance liquid chromatography separations of small molecules in isocratic mode. J Chromatogr A 1228:250-62
Lv, Yongqin; Alejandro, Fernando Maya; Fr├ęchet, Jean M J et al. (2012) Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles. J Chromatogr A 1261:121-8
Urban, Jiri; Svec, Frantisek; Frechet, Jean M J (2012) A monolithic lipase reactor for biodiesel production by transesterification of triacylglycerides into fatty acid methyl esters. Biotechnol Bioeng 109:371-80
Chambers, Stuart D; Svec, Frantisek; Frechet, Jean M J (2011) Incorporation of carbon nanotubes in porous polymer monolithic capillary columns to enhance the chromatographic separation of small molecules. J Chromatogr A 1218:2546-52
Chambers, Stuart D; Holcombe, Thomas W; Svec, Frantisek et al. (2011) Porous polymer monoliths functionalized through copolymerization of a C60 fullerene-containing methacrylate monomer for highly efficient separations of small molecules. Anal Chem 83:9478-84
Teisseyre, Thomas Z; Urban, Jiri; Halpern-Manners, Nicholas W et al. (2011) Remotely detected NMR for the characterization of flow and fast chromatographic separations using organic polymer monoliths. Anal Chem 83:6004-10
Walsh, Zarah; Levkin, Pavel A; Jain, Vijay et al. (2010) Visible light initiated polymerization of styrenic monolithic stationary phases using 470 nm light emitting diode arrays. J Sep Sci 33:61-6

Showing the most recent 10 out of 74 publications