Abstract

In the first grant period a deletion of the clathrin heavy chain was constructed. The investigator proposes to take advantage of the clathrin null cells to explore the structural details of clathrin assembly. A variety of truncations will be introduced into the nulls cells and the assembled clathrin analyzed for structural and functional changes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM048625-08
Application #
6138455
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Shapiro, Bert I
Project Start
1994-01-01
Project End
2001-12-31
Budget Start
2000-01-01
Budget End
2000-12-31
Support Year
8
Fiscal Year
2000
Total Cost
$259,154
Indirect Cost

  Institution

Name
University of Texas Austin
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
City
Austin
State
TX
Country
United States
Zip Code
78712

  Publications

Sosa, R Thomas; Weber, Michelle M; Wen, Yujia et al. (2012) A single ? adaptin contributes to AP1 and AP2 complexes and clathrin function in Dictyostelium. Traffic 13:305-16
Brady, Rebecca J; Damer, Cynthia K; Heuser, John E et al. (2010) Regulation of Hip1r by epsin controls the temporal and spatial coupling of actin filaments to clathrin-coated pits. J Cell Sci 123:3652-61
Wen, Yujia; Stavrou, Irene; Bersuker, Kirill et al. (2009) AP180-mediated trafficking of Vamp7B limits homotypic fusion of Dictyostelium contractile vacuoles. Mol Biol Cell 20:4278-88
Brady, Rebecca J; Wen, Yujia; O'Halloran, Theresa J (2008) The ENTH and C-terminal domains of Dictyostelium epsin cooperate to regulate the dynamic interaction with clathrin-coated pits. J Cell Sci 121:3433-44
Repass, Shannon Lea; Brady, Rebecca J; O'Halloran, Theresa J (2007) Dictyostelium Hip1r contributes to spore shape and requires epsin for phosphorylation and localization. J Cell Sci 120:3977-88
Wang, Jingshan; Wang, Yanqin; O'Halloran, Theresa J (2006) Clathrin light chain: importance of the conserved carboxy terminal domain to function in living cells. Traffic 7:824-32
Stavrou, Irene; O'Halloran, Theresa J (2006) The monomeric clathrin assembly protein, AP180, regulates contractile vacuole size in Dictyostelium discoideum. Mol Biol Cell 17:5381-9
Wang, Yanqin; O'Halloran, Theresa J (2006) Abp1 regulates pseudopodium number in chemotaxing Dictyostelium cells. J Cell Sci 119:702-10
Wang, Jingshan; Virta, Valerie C; Riddelle-Spencer, Kathryn et al. (2003) Compromise of clathrin function and membrane association by clathrin light chain deletion. Traffic 4:891-901
Norian, L; Dragoi, I A; O'Halloran, T (1999) Molecular characterization of rabE, a developmentally regulated Dictyostelium homolog of mammalian rab GTPases. DNA Cell Biol 18:59-64

Showing the most recent 10 out of 12 publications