Crystallographic analysis will be carried out on two DNA polymerases of the """"""""Pol I"""""""" family, E. coli DNA polymerase I and Thermus aquaticus (Taq) DNA polymerase, and Pol alpha class DNA polymerases from bacteriophages T4 and RB69.
The aim i s to understand the mechanism of fidelity in structural terms, and towards this end, the structures of template-primer and dNTP complexes will be determined. For the bacteriophage proteins, the structures of complexes of the polymerase with single stranded DNA binding protein and the processivity factor will be pursued.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM057510-03
Application #
6180554
Study Section
Molecular and Cellular Biophysics Study Section (BBCA)
Program Officer
Lewis, Catherine D
Project Start
1998-07-01
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
3
Fiscal Year
2000
Total Cost
$198,070
Indirect Cost
Name
Yale University
Department
Biochemistry
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520
Zuo, Yuhong; Steitz, Thomas A (2015) Crystal structures of the E. coli transcription initiation complexes with a complete bubble. Mol Cell 58:534-40
Liu, Bin; Zuo, Yuhong; Steitz, Thomas A (2015) Structural basis for transcription reactivation by RapA. Proc Natl Acad Sci U S A 112:2006-10
Ritacco, Christopher J; Steitz, Thomas A; Wang, Jimin (2014) Exploiting large non-isomorphous differences for phase determination of a G-segment invertase-DNA complex. Acta Crystallogr D Biol Crystallogr 70:685-93
Ritacco, Christopher J; Kamtekar, Satwik; Wang, Jimin et al. (2013) Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase. Nucleic Acids Res 41:2673-82
Liu, Bin; Eliason, William K; Steitz, Thomas A (2013) Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly. Nat Commun 4:2495
Zuo, Yuhong; Wang, Yeming; Steitz, Thomas A (2013) The mechanism of E. coli RNA polymerase regulation by ppGpp is suggested by the structure of their complex. Mol Cell 50:430-6
Liu, Bin; Lin, Jinzhong; Steitz, Thomas A (2013) Structure of the PolIII?-?c-DNA complex suggests an atomic model of the replisome. Structure 21:658-64
Itsathitphaisarn, Ornchuma; Wing, Richard A; Eliason, William K et al. (2012) The hexameric helicase DnaB adopts a nonplanar conformation during translocation. Cell 151:267-77
Wang, Mina; Xia, Shuangluo; Blaha, Gregor et al. (2011) Insights into base selectivity from the 1.8 Å resolution structure of an RB69 DNA polymerase ternary complex. Biochemistry 50:581-90
Pan, Baocheng; Xiong, Yong; Steitz, Thomas A (2010) How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA. Science 330:937-40

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