The long-term goals of our laboratory are to understand though genetic analysis the evolutionary forces producing new species and species differences in behavioral and morphological traits, to identify the genes involved in such differences and, ultimately, to determine if there are general patterns in the genetics of speciation. We propose to study these questions in two pairs of recently-diverged sister species in the Drosophila melanogaster subgroup. Our specific questions are the following: 1. What are genetic bases of hybrid male sterility and morphological and behavioral differences between D. santomea and D. yakuba? These species show strong sexual isolation and hybrid male sterility, as well as strong differences in abdominal pigmentation and male courtship song. We propose to use fine-structure molecular mapping to derive a small set of candidate genes affecting pigmentation and hybrid sterility, as well as an initial genome-wide QTL mapping study of an interspecific difference in male mating song that may play a role in sexual isolation. 2. What are the genes responsible for sexual isolation between D. simulans and D. mauritiana? Our QTL mapping of sexual isolation between these two species has revealed several small regions of the genome affecting mate discrimination. We propose to localize """"""""sexual isolation"""""""" genes more accurately in both sexes using fine-structure genetic mapping. 3. How much introgression is permissible among species? We propose to determine how much mixing between separately-evolved species'genomes is permissible by constructing laboratory 'hybrid swarms'between pairs of closely-related species, and following the evolution of molecularly marked chromosome segments, diagnostic morphological traits, and sexual isolation over several years. 4. What explains anomalies in the """"""""hybrid zone"""""""" and genetic composition of hybrids in the wild? Naturally- occurring hybrids found on Sao Tome Island are peculiar: they are all male, all F1 hybrids having a D. santomea mother, and nearly all of them occur at high altitude outside the range of either parental species. We will perform laboratory studies of tolerance and preference to understand these anomalies. Our results may be relevant to understanding the origin of species in other animal groups, including humans.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Project (R01)
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Genetic Variation and Evolution Study Section (GVE)
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Eckstrand, Irene A
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University of Chicago
Schools of Medicine
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Matute, Daniel R; Butler, Ian A; Turissini, David A et al. (2010) A test of the snowball theory for the rate of evolution of hybrid incompatibilities. Science 329:1518-21
Matute, Daniel R (2010) Reinforcement of gametic isolation in Drosophila. PLoS Biol 8:e1000341
Matute, Daniel R (2010) Reinforcement can overcome gene flow during speciation in Drosophila. Curr Biol 20:2229-33
Matute, Daniel R; Coyne, Jerry A (2010) Intrinsic reproductive isolation between two sister species of Drosophila. Evolution 64:903-20
Matute, Daniel R; Novak, Caroline J; Coyne, Jerry A (2009) Temperature-based extrinsic reproductive isolation in two species of Drosophila. Evolution 63:595-612
Matute, Daniel R; Butler, Ian A; Coyne, Jerry A (2009) Little effect of the tan locus on pigmentation in female hybrids between Drosophila santomea and D. melanogaster. Cell 139:1180-8
Coyne, Jerry A; Kay, Emily H; Pruett-Jones, Stephen (2008) The genetic basis of sexual dimorphism in birds. Evolution 62:214-9
Hoekstra, Hopi E; Coyne, Jerry A (2007) The locus of evolution: evo devo and the genetics of adaptation. Evolution 61:995-1016
Coyne, Jerry A; Elwyn, Susannah (2006) Does the desaturase-2 locus in Drosophila melanogaster cause adaptation and sexual isolation? Evolution 60:279-91
Edwards, Alexis C; Rollmann, Stephanie M; Morgan, Theodore J et al. (2006) Quantitative genomics of aggressive behavior in Drosophila melanogaster. PLoS Genet 2:e154

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