A structural analysis of the focal contact protein vinculin is proposed. Using a combination of X-ray crystallography and NMR, it is proposed to determine atomic models of full-length vinculin as well as isolated domains, and then determine the structures of complexes with binding-protein fragments. These data will be used to map out binding sites which, by reference to the structure of the intact molecule, will also suggest how binding is regulated. Using this information, mutants lacking specific functions will be generated and their ability to rescue vinculin null cells assessed in vivo.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM059760-02
Application #
6182045
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Deatherage, James F
Project Start
1999-08-01
Project End
2003-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
2
Fiscal Year
2000
Total Cost
$356,948
Indirect Cost
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
009214214
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Ziegler, Wolfgang H; Liddington, Robert C; Critchley, David R (2006) The structure and regulation of vinculin. Trends Cell Biol 16:453-60