The focus of this renewal application is the continued development and application of a unique multiscale approach to study membranes and membrane-bound processes. The project involves the development of conceptual and computational methodologies for three interconnected regimes at three different scales, i.e., the continuum, mesoscopic, and atomistic scales. Generally speaking, these three regimes are separated by up to three orders-of-magnitude in their relevant length- and time-scales. In this project, new approaches will continue to be developed for each of the three regimes (especially at the challenging continuum and mesoscopic scales) and, importantly, they will be linked together in a multi-scale computational fashion. The resulting Specific Aims will involve: (1) the development of a multi-scale simulation approach for the description of membrane bound proteins and peptides, with specific applications to mechanosensitive channels that are gated in response to external applied stress, BAR domain-containing proteins which are believed to induce membrane curvature, and neuroactive peptides that affect ion channel function; (2) the coupling of the mesoscopic simulation component to NMR measurements in order to both validate the next generation mesoscopic model and to unravel the origins of complex, experimentally observed mesoscopic and atomistic-level membrane motions; and (3) the development of a continuum-level description of lipid domain formation and dynamics coupled to large-scale membrane motion and deformation, through a novel synthesis of field theory with computational continuum mechanics. The overarching long term goal of this project is to provide a new multi-scale, physics-based approach for the computational study of biologically important membrane and membrane-bound processes. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM063796-06
Application #
7113685
Study Section
Special Emphasis Panel (ZRG1-BCMB-Q (02))
Program Officer
Chin, Jean
Project Start
2001-06-01
Project End
2009-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
6
Fiscal Year
2006
Total Cost
$277,375
Indirect Cost
Name
University of Utah
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Madsen, Jesper J; Grime, John M A; Rossman, Jeremy S et al. (2018) Entropic forces drive clustering and spatial localization of influenza A M2 during viral budding. Proc Natl Acad Sci U S A 115:E8595-E8603
Simunovic, Mijo; Bassereau, Patricia; Voth, Gregory A (2018) Organizing membrane-curving proteins: the emerging dynamical picture. Curr Opin Struct Biol 51:99-105
Davtyan, Aram; Simunovic, Mijo; Voth, Gregory A (2017) The mesoscopic membrane with proteins (MesM-P) model. J Chem Phys 147:044101
Simunovic, Mijo; Manneville, Jean-Baptiste; Renard, Henri-François et al. (2017) Friction Mediates Scission of Tubular Membranes Scaffolded by BAR Proteins. Cell 170:172-184.e11
Simunovic, Mijo; Šari?, An?ela; Henderson, J Michael et al. (2017) Long-Range Organization of Membrane-Curving Proteins. ACS Cent Sci 3:1246-1253
Simunovic, Mijo; Evergren, Emma; Golushko, Ivan et al. (2016) How curvature-generating proteins build scaffolds on membrane nanotubes. Proc Natl Acad Sci U S A 113:11226-11231
Davtyan, Aram; Simunovic, Mijo; Voth, Gregory A (2016) Multiscale simulations of protein-facilitated membrane remodeling. J Struct Biol 196:57-63
Simunovic, Mijo; Voth, Gregory A (2015) Membrane tension controls the assembly of curvature-generating proteins. Nat Commun 6:7219
Simunovic, Mijo; Voth, Gregory A; Callan-Jones, Andrew et al. (2015) When Physics Takes Over: BAR Proteins and Membrane Curvature. Trends Cell Biol 25:780-792
Li, Jianing; Ziemba, Brian P; Falke, Joseph J et al. (2014) Interactions of protein kinase C-? C1A and C1B domains with membranes: a combined computational and experimental study. J Am Chem Soc 136:11757-66

Showing the most recent 10 out of 52 publications