In this Administrative Supplement for Equipment application, Dr. Tatyana Svitkina and I request funds to purchase LUNV 7-line Laser Launch from Nikon Instruments Inc. to fully functionalize our shared fluorescence microscope Nikon TiE. The parent project focuses on the molecular mechanisms that control the biogenesis and release of the exosomes, and their role in cancer cell invasion. Advanced microscopy is essential for the accomplishment of goals proposed in the grant. The requested equipment will enable our microscopy system with a capability to perform multicolor imaging using both spinning disk confocal and total internal reflection fluorescence modalities using a high-end integrated laser system that does not require calibration and alignments of lasers. This equipment will replace our current separate low-end laser launches, one of which is obsolete and unreliable. The requested equipment is needed to achieve goals of the parent grant ?Exosome Trafficking and Tumor Invasion? (R01 GM085146).

Public Health Relevance

Exosomes play important role in many pathophysiological processes such as tumor invasion and cancer metastasis. Studying the molecular basis and regulatory mechanisms of exosomal trafficking, and how exosomes promote tumor invasion will help us understand many basic physiological processes and bridge the fields of basic exosome biology and cancer biology. Fluorescence microscopy is an essential tool for this project.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM085146-11S1
Application #
9891306
Study Section
Nuclear and Cytoplasmic Structure/Function and Dynamics Study Section (NCSD)
Program Officer
Xu, Jianhua
Project Start
2009-06-01
Project End
2021-04-30
Budget Start
2019-05-01
Budget End
2020-04-30
Support Year
11
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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Lu, Hezhe; Liu, Shujing; Zhang, Gao et al. (2016) Oncogenic BRAF-Mediated Melanoma Cell Invasion. Cell Rep 15:2012-24
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Lu, Hezhe; Liu, Jianglan; Liu, Shujing et al. (2013) Exo70 isoform switching upon epithelial-mesenchymal transition mediates cancer cell invasion. Dev Cell 27:560-73
Cai, Bishuang; Giridharan, Sai Srinivas Panapakkam; Zhang, Jing et al. (2013) Differential roles of C-terminal Eps15 homology domain proteins as vesiculators and tubulators of recycling endosomes. J Biol Chem 288:30172-80

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