The objective of this project is to understand the cellular mechanisms of the maternal/fetal transfer of important nutrients by the placenta. The proposed Aims involve 1) the cloning of the cDNAs for the trophoblast protein species mediating cationic amino acid transport; 2) the characterization of their functional capacities in an expression system to compare their activities with those of placental membranes; 3) the development and use of antibodies to the cloned transporter proteins for the quantitative assessment of transporter protein in microvillous and basal membranes, as well as other placental sources; and 4) the understanding of transporter regulation during trophoblast differentiation and membrane specialization.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
5R01HD007562-26
Application #
6182001
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Program Officer
Ilekis, John V
Project Start
1977-03-01
Project End
2003-02-28
Budget Start
2000-09-01
Budget End
2003-02-28
Support Year
26
Fiscal Year
2000
Total Cost
$286,112
Indirect Cost
Name
Washington University
Department
Pediatrics
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130