Recently it has become apparent that the egg plasma membrane is modified during fertilization and that these changes may play a role in the activation of egg metabolism and development. The objective of this project is to characterize the integral and peripheral proteins of the sea urchin egg plasma membrane, determine how they are modified at fertilization and evaluate the role of cortical vesicle exocytosis in bringing about these changes. The project will involve the following: 1) Further characterization of the egg plasma membrane proteins by selective extraction procedures and radiolabelling techniques. 2) Isolation of the cortical secretory vesicles and characterization of the soluble and membrane bound components. 3) Isolation of the plasma membrane from fertilized eggs and evaluation of the changes in polypeptide organization and content which occur at fertilization and 4) Determination of the role that the cortical vesicles play in these changes, including incorporation of vesicle components into the plasma membrane and enzymatic modification of cell surface proteins. In addition to basic information on the egg plasma membrane and its interaction with the cortical secretory vesicles, this work will serve as a basis for future investigations of the role of these cell surface changes in regulating metabolism and development.
| Luo, Jinping; Gupta, Vijayalaxmi; Kern, Brian et al. (2012) Role of FYN kinase in spermatogenesis: defects characteristic of Fyn-null sperm in mice. Biol Reprod 86:1-8 |
| Jasti, Susmita; Warren, Bryce D; McGinnis, Lynda K et al. (2012) The autoimmune regulator prevents premature reproductive senescence in female mice. Biol Reprod 86:110 |
| McGinnis, Lynda K; Hong, Xiaoman; Christenson, Lane K et al. (2011) Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes. Mol Reprod Dev 78:33-47 |
| McGinnis, Lynda K; Carroll, David J; Kinsey, William H (2011) Protein tyrosine kinase signaling during oocyte maturation and fertilization. Mol Reprod Dev 78:831-45 |
| Luo, Jinping; McGinnis, Lynda K; Kinsey, William H (2010) Role of Fyn kinase in oocyte developmental potential. Reprod Fertil Dev 22:966-76 |
| Luo, Jinping; McGinnis, Lynda K; Kinsey, William H (2009) Fyn kinase activity is required for normal organization and functional polarity of the mouse oocyte cortex. Mol Reprod Dev 76:819-31 |
| Kinsey, William H (2009) Analysis of signaling pathways in zebrafish development by microinjection. Methods Mol Biol 518:67-76 |
| McGinnis, Lynda K; Kinsey, William H; Albertini, David F (2009) Functions of Fyn kinase in the completion of meiosis in mouse oocytes. Dev Biol 327:280-7 |
| Sharma, Dipika; Kinsey, William H (2008) Regionalized calcium signaling in zebrafish fertilization. Int J Dev Biol 52:561-70 |
| McGinnis, Lynda K; Albertini, David F; Kinsey, William H (2007) Localized activation of Src-family protein kinases in the mouse egg. Dev Biol 306:241-54 |
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