Abstract

Aim 1 is to delineate the role of changes in subunit gene transcription rates in the expression of gonadotropin subunits, GnRH receptor (GnRH-R) and activin beta-B mRNAs.
Aim 2 is to investigate the intracellular (3rd) messenger mechanisms that mediate GnRH/Ca pulse frequency regulation of differential expression of gonadotrope genes and will: 1) assess the effects of blockade of PKA, PKC, CAMKII, and MAPK on transcriptional and mRNA responses different frequency GnRH or Ca pulses; 2) assess activation of PKA, PKC, CAMKII, and MAPKs after Ca or GnRH pulses.
Aim 3 will investigate the MAPK pathways (ERK, JNK, p38) involved in GnRH stimulation of gonadotrope gene expression.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Project (R01)
Project #
2R01HD033039-05
Application #
6192995
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
De Paolo, Louis V
Project Start
2000-08-15
Project End
2004-07-31
Budget Start
2000-08-15
Budget End
2001-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$266,400
Indirect Cost

  Institution

Name
University of Virginia
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Abshire, Michelle Y; Blank, Susan K; Chhabra, Sandhya et al. (2012) Role of androgen receptor CAG repeat polymorphism length in hypothalamic progesterone sensitivity in hyperandrogenic adolescent girls. Endocrine 41:156-8
Burger, Laura L; Haisenleder, Daniel J; Marshall, John C (2011) GnRH pulse frequency differentially regulates steroidogenic factor 1 (SF1), dosage-sensitive sex reversal-AHC critical region on the X chromosome gene 1 (DAX1), and serum response factor (SRF): potential mechanism for GnRH pulse frequency regulation of LH Endocrine 39:212-9
Burger, Laura L; Haisenleder, Daniel J; Aylor, Kevin W et al. (2009) Regulation of Lhb and Egr1 gene expression by GNRH pulses in rat pituitaries is both c-Jun N-terminal kinase (JNK)- and extracellular signal-regulated kinase (ERK)-dependent. Biol Reprod 81:1206-15
Burger, Laura L; Haisenleder, Daniel J; Aylor, Kevin W et al. (2008) Regulation of intracellular signaling cascades by GNRH pulse frequency in the rat pituitary: roles for CaMK II, ERK, and JNK activation. Biol Reprod 79:947-53
Haisenleder, D J; Burger, L L; Walsh, H E et al. (2008) Pulsatile gonadotropin-releasing hormone stimulation of gonadotropin subunit transcription in rat pituitaries: evidence for the involvement of Jun N-terminal kinase but not p38. Endocrinology 149:139-45
Blank, Susan K; McCartney, Christopher R; Helm, Kristen D et al. (2007) Neuroendocrine effects of androgens in adult polycystic ovary syndrome and female puberty. Semin Reprod Med 25:352-9
Burger, Laura L; Haisenleder, Daniel J; Wotton, Gordon M et al. (2007) The regulation of FSHbeta transcription by gonadal steroids: testosterone and estradiol modulation of the activin intracellular signaling pathway. Am J Physiol Endocrinol Metab 293:E277-85
Blank, S K; McCartney, C R; Marshall, J C (2006) The origins and sequelae of abnormal neuroendocrine function in polycystic ovary syndrome. Hum Reprod Update 12:351-61
Haisenleder, D J; Burger, L L; Aylor, K W et al. (2005) Testosterone stimulates follicle-stimulating hormone beta transcription via activation of extracellular signal-regulated kinase: evidence in rat pituitary cells. Biol Reprod 72:523-9
Prendergast, Kathleen A; Burger, Laura L; Aylor, Kevin W et al. (2004) Pituitary follistatin gene expression in female rats: evidence that inhibin regulates transcription. Biol Reprod 70:364-70

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