Abstract

This project focuses on the human Y chromosome in the context of the Human Genome Initiative. The principal long-term goal of this project is complete definition of the genetic content of the human Y chromosome. Toward that end, a detailed genetic deletion map of the chromosome will be constructed, overlapping recombinant DNA clones spanning the chromosome will be isolated, and the nucleotide sequence of the chromosome will be determined. During the early course of this work, high priority will be given to the development of efficient DNA sequencing strategies using automated instruments. The resulting information will provide a framework for elucidating the function of the Y chromosomes in processes as diverse as spermatogenesis, tumorigenesis, and embryonic somatic and sexual development. These studies will also reveal much about the complex functional, structural, and evolutionary relationship between the human X and Y chromosomes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Project (R01)
Project #
1R01HG000257-01
Application #
3333323
Study Section
Genome Study Section (GNM)
Project Start
1991-01-01
Project End
1993-12-31
Budget Start
1991-01-01
Budget End
1991-12-31
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Whitehead Institute for Biomedical Research
Department
Type
DUNS #
076580745
City
Cambridge
State
MA
Country
United States
Zip Code
02142

  Publications

Bellott, Daniel W; Skaletsky, Helen; Cho, Ting-Jan et al. (2017) Avian W and mammalian Y chromosomes convergently retained dosage-sensitive regulators. Nat Genet 49:387-394
Hughes, Jennifer F; Skaletsky, Helen; Koutseva, Natalia et al. (2015) Sex chromosome-to-autosome transposition events counter Y-chromosome gene loss in mammals. Genome Biol 16:104
Soh, Y Q Shirleen; Alföldi, Jessica; Pyntikova, Tatyana et al. (2014) Sequencing the mouse Y chromosome reveals convergent gene acquisition and amplification on both sex chromosomes. Cell 159:800-13
Peterson, Julie A; Gitter, Maria; Bougie, Daniel W et al. (2014) Low-frequency human platelet antigens as triggers for neonatal alloimmune thrombocytopenia. Transfusion 54:1286-93
Okumura, Leah M; Lesch, Bluma J; Page, David C (2013) The ligand binding domain of GCNF is not required for repression of pluripotency genes in mouse fetal ovarian germ cells. PLoS One 8:e66062
Wang, Haoyi; Hu, Yueh-Chiang; Markoulaki, Styliani et al. (2013) TALEN-mediated editing of the mouse Y chromosome. Nat Biotechnol 31:530-2
Mueller, Jacob L; Skaletsky, Helen; Brown, Laura G et al. (2013) Independent specialization of the human and mouse X chromosomes for the male germ line. Nat Genet 45:1083-7
Lange, Julian; Noordam, Michiel J; van Daalen, Saskia K M et al. (2013) Intrachromosomal homologous recombination between inverted amplicons on opposing Y-chromosome arms. Genomics 102:257-64
Hughes, Jennifer F; Skaletsky, Helen; Brown, Laura G et al. (2012) Strict evolutionary conservation followed rapid gene loss on human and rhesus Y chromosomes. Nature 483:82-6
Hughes, Jennifer F; Skaletsky, Helen; Page, David C (2012) Sequencing of rhesus macaque Y chromosome clarifies origins and evolution of the DAZ (Deleted in AZoospermia) genes. Bioessays 34:1035-44

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