The aims of the research proposed here are to study the structure and function of several membrane proteins in eukaryotic cells: the Band 3 protein of human red blood cells; the (Na+,K+) ATPase of a variety of cells; the Ca++ ATPase of liver plasma membrane; and the Na+/Ca++ exchange protein of cardiac sarcolemma and brain synaptosomes.
One aim i s to obtain a map showing the arrangement of each polypeptide with respect to the lipid bilayer. Another aim is to study the protein-lipid interactions, the importance of the oligomeric structure, the mechanism of action, and the regulation of these transporters.
|Paavilainen, Sari; Guidotti, Guido (2014) Interactions between the transmembrane domains of CD39: identification of interacting residues by yeast selection. ScienceOpen Res 2014:|
|Grinthal, Alison; Guidotti, Guido (2007) Bilayer mechanical properties regulate the transmembrane helix mobility and enzymatic state of CD39. Biochemistry 46:279-90|