This application seeks to continue 20 years of support focusing on the microvascular, neuronal somatic, axonal and deafferentation-mediated responses to traumatic brain injury. In previous funding periods, we have shown that the injury does not tear axons. Rather, it triggers local axonal damage that leads to continued alteration and ultimate disconnection. It has been assumed by all that once disconnected the proximal axonal tip swells, due to the delivery of substances via anterograde transport, with the resulting axonal bulb formation becoming the universally recognized endpoint for all contemporary forensic, neuropathological and experimental studies. Recently, our laboratory has suggested that many injured axons may not progress to bulb formation, which suggests differing modes of pathogenesis and potential therapeutic modulation. In this application we focus on this issue using different animal models of TBI, employing lissencephalic and gyrencephalic species. The resulting TAI will be followed over time by double label immunocytochemical strategies targeting various cytoskeletal, axolemmal and axonal transport abnormalities. Quantitative, computer-assisted EM analysis will be used to better understand the precise subcellular changes associated with TIA as well as the mechanisms related thereto. Moving on the premise that we will observe different populations of injured axons, with differing forms of pathogenesis, we will also pursue descriptive and mechanistic studies related to their response to agents reported to attenuate TAI. Cyclosporin A and FK506 will be used in addition to hypothermia to assess their impact on these differing forms of TAI via the same strategies noted above. These studies will be interfaced with electrophysiological and neurochemical assessments to examine any therapeutic modulation of action potentials, calcineurin activity or mitochondrial function. The successful conduct of these studies should provide new insight into the complex pathobiology of TAI and its therapeutic modulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS020193-23
Application #
6986231
Study Section
Special Emphasis Panel (ZRG1-BDCN-1 (01))
Program Officer
Hicks, Ramona R
Project Start
1983-12-01
Project End
2007-11-30
Budget Start
2005-12-01
Budget End
2007-11-30
Support Year
23
Fiscal Year
2006
Total Cost
$486,477
Indirect Cost
Name
Virginia Commonwealth University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298
Lifshitz, Jonathan; Kelley, Brian Joseph; Povlishock, John Theodore (2007) Perisomatic thalamic axotomy after diffuse traumatic brain injury is associated with atrophy rather than cell death. J Neuropathol Exp Neurol 66:218-29
Marmarou, Christina R; Povlishock, John T (2006) Administration of the immunophilin ligand FK506 differentially attenuates neurofilament compaction and impaired axonal transport in injured axons following diffuse traumatic brain injury. Exp Neurol 197:353-62
Tamas, Andrea; Zsombok, Andrea; Farkas, Orsolya et al. (2006) Postinjury administration of pituitary adenylate cyclase activating polypeptide (PACAP) attenuates traumatically induced axonal injury in rats. J Neurotrauma 23:686-95
Ueda, Yuji; Walker, Susan A; Povlishock, John T (2006) Perivascular nerve damage in the cerebral circulation following traumatic brain injury. Acta Neuropathol 112:85-94
Buki, A; Povlishock, J T (2006) All roads lead to disconnection?--Traumatic axonal injury revisited. Acta Neurochir (Wien) 148:181-93; discussion 193-4
Marmarou, Christina R; Walker, Susan A; Davis, C Lynn et al. (2005) Quantitative analysis of the relationship between intra- axonal neurofilament compaction and impaired axonal transport following diffuse traumatic brain injury. J Neurotrauma 22:1066-80
Jackson, Tanisha A; Taylor, Harry E; Sharma, Deva et al. (2005) Vascular endothelial growth factor receptor-2: counter-regulation by the transcription factors, TFII-I and TFII-IRD1. J Biol Chem 280:29856-63
Reeves, Thomas M; Phillips, Linda L; Povlishock, John T (2005) Myelinated and unmyelinated axons of the corpus callosum differ in vulnerability and functional recovery following traumatic brain injury. Exp Neurol 196:126-37
Ueda, Yuji; Suehiro, Eiichi; Wei, Enoch P et al. (2004) Uncomplicated rapid posthypothermic rewarming alters cerebrovascular responsiveness. Stroke 35:601-6
Reeves, Thomas M; Prins, Mayumi L; Zhu, JiePei et al. (2003) Matrix metalloproteinase inhibition alters functional and structural correlates of deafferentation-induced sprouting in the dentate gyrus. J Neurosci 23:10182-9

Showing the most recent 10 out of 62 publications