Tsetse flies transmit the human and animal forms of African Trypanosomiasis, neglected diseases, which affect marginalized populations in sub-Saharan Africa. Control methods based upon pesticide-baited targets and traps can reduce tsetse populations, but have downsides, including environmental toxicity, collateral effects upon non- target organisms and high implementation cost. Thus, genetic and non-pesticide based vector control methods are desirable. This project will build the foundation for control methods that aim to reduce tsetse's reproductive capacity. We will investigate male accessory gland (MAGs) products, known as seminal fluid proteins (SFPs), which are transferred along with sperm to the female during mating. SFPs mediate important physiological and behavioral changes in mated female insects, including reduced receptivity to remating and increased oogenesis/egg deposition. Disruption/manipulation of SFP function(s) has the potential to reduce tsetse populations and hence disease transmission. Tsetse flies have a low reproductive capacity as they reproduce by obligate viviparity. Each gonotrophic cycle, females carry and nourish a single intrauterine offspring for the duration of its development. Females on average produce 8-10 offspring per lifetime. Our proposed studies are supported by the availability of 1) preliminary transcriptomic data on MAG gene expression in our target species Glossina morsitans morsitans (Gmm), 2) an annotated genome and extensive transcriptomic resources for Gmm, and 3) a large dataset of transcriptomes from other tsetse species. This study has 3 aims: 1. Identify putative SFP genes using transcriptomic, proteomic and genomic approaches, characterize their sex/tissue specificity, and examine temporal expression profiles during maturation and after mating. 2. Identify SFP orthologs and paralogs between multiple Glossina species by utilizing available transcriptomic and genomic resources, estimate sequence diversity of SFPs by polymorphism detection and identify conserved sex and tissue specific regulatory elements by in silico analysis. 3. Develop and apply gene silencing techniques to assess the functional role(s) of selected SFPs (~5) within the context of the tsetse reproductive fitness parameters.

Public Health Relevance

The male seminal fluid proteins of flies are important modulators of female fertility, physiology and behavior after mating occurs. We will identify male tsetse seminal fluid proteins and characterize the role that these proteins play in the regulation of various aspects of the female tsetse fly's reproductive cycle. This information will be importan to the development of novel reproduction based vector control strategies aimed at preventing Trypanosomiasis transmission.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Exploratory/Developmental Grants (R21)
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Vector Biology Study Section (VB)
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Costero, Adriana
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Yale University
Public Health & Prev Medicine
Schools of Medicine
New Haven
United States
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Benoit, Joshua B; Michalkova, Veronika; Didion, Elise M et al. (2018) Rapid autophagic regression of the milk gland during involution is critical for maximizing tsetse viviparous reproductive output. PLoS Negl Trop Dis 12:e0006204
Scolari, Francesca; Attardo, Geoffrey Michael; Aksoy, Emre et al. (2018) Symbiotic microbes affect the expression of male reproductive genes in Glossina m. morsitans. BMC Microbiol 18:169
Bing, XiaoLi; Attardo, Geoffrey M; Vigneron, Aurelien et al. (2017) Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks. Proc Biol Sci 284:
Scolari, Francesca; Benoit, Joshua B; Michalkova, Veronika et al. (2016) The Spermatophore in Glossina morsitans morsitans: Insights into Male Contributions to Reproduction. Sci Rep 6:20334
Benoit, Joshua B; Attardo, Geoffrey M; Baumann, Aaron A et al. (2015) Adenotrophic viviparity in tsetse flies: potential for population control and as an insect model for lactation. Annu Rev Entomol 60:351-71
Shaw, W Robert; Attardo, Geoffrey M; Aksoy, Serap et al. (2015) A comparative analysis of reproductive biology of insect vectors of human disease. Curr Opin Insect Sci 10:142-148
Benoit, Joshua B; Attardo, Geoffrey M; Michalkova, Veronika et al. (2014) A novel highly divergent protein family identified from a viviparous insect by RNA-seq analysis: a potential target for tsetse fly-specific abortifacients. PLoS Genet 10:e1003874
Attardo, Geoffrey M; Benoit, Joshua B; Michalkova, Veronika et al. (2014) The homeodomain protein ladybird late regulates synthesis of milk proteins during pregnancy in the tsetse fly (Glossina morsitans). PLoS Negl Trop Dis 8:e2645
Benoit, Joshua B; Hansen, Immo A; Attardo, Geoffrey M et al. (2014) Aquaporins are critical for provision of water during lactation and intrauterine progeny hydration to maintain tsetse fly reproductive success. PLoS Negl Trop Dis 8:e2517
Michalkova, Veronika; Benoit, Joshua B; Attardo, Geoffrey M et al. (2014) Amelioration of reproduction-associated oxidative stress in a viviparous insect is critical to prevent reproductive senescence. PLoS One 9:e87554

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