The vision research faculty in the Department of Ophthalmology at the University of Missouri propose to establish two research modules to support the ongoing activities in the department. The Protein expression and analysis module (Module #1) will be setup under the direction of Dr. Sharma for crystallin expression, isolation, and analysis. This module will support the need of investigators who will need large quantities of crystallins, need assistance in the analysis of lens or retinal constituents and the need to culture of whole lenses or lens epithelial cells. The establishment of lens epithelial cells in culture will allow us to look into signal transduction and gene expression under in vivo conditions. The establishment of protein analysis module will allow us to share the existing resources and increase our ability to offer support to recently joined faculty and the new faulty we plan on recruiting under the mission enhancement program. The Histology and image analysis module (Module #2) will be a facility for analyzing eye tissue samples from transgenic animals or pathologic specimens or donor tissues. This module will be directed by Dr. Reneker. It will foster the ongoing collaboration between the NEI supported projects within the department. In addition, the module will offer the much needed support for the research projects initiated by our new faculty. Therefore the main objectives of this proposal are to: a) enhance research infrastructure for vision science research at University of Missouri School of Medicine, b) enhance the opportunities for joint research projects and provide logistical support for joint projects, c) support the needs of new vision research faculty.

National Institute of Health (NIH)
National Eye Institute (NEI)
Resource-Related Research Projects (R24)
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Study Section
Special Emphasis Panel (ZEY1-VSN (08))
Program Officer
Chin, Hemin R
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University of Missouri-Columbia
Schools of Medicine
United States
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Zhang, Jinglin; Upadhya, Dinesh; Lu, Lin et al. (2015) Fibroblast growth factor receptor 2 (FGFR2) is required for corneal epithelial cell proliferation and differentiation during embryonic development. PLoS One 10:e0117089
Upadhya, Dinesh; Ogata, Masato; Reneker, Lixing W (2013) MAPK1 is required for establishing the pattern of cell proliferation and for cell survival during lens development. Development 140:1573-82
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Reneker, Lixing W; Bloch, Amy; Xie, Leike et al. (2010) Induction of corneal myofibroblasts by lens-derived transforming growth factor beta1 (TGFbeta1): a transgenic mouse model. Brain Res Bull 81:287-96
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Murugesan, Raju; Santhoshkumar, Puttur; Sharma, K Krishna (2008) Role of alphaBI5 and alphaBT162 residues in subunit interaction during oligomerization of alphaB-crystallin. Mol Vis 14:1835-44
Maddala, Rupalatha; Reneker, Lixing W; Pendurthi, Bhavana et al. (2008) Rho GDP dissociation inhibitor-mediated disruption of Rho GTPase activity impairs lens fiber cell migration, elongation and survival. Dev Biol 315:217-31

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