? This is a request for a three-year supplement to our ongoing Center grant, in order to meet the increased need to build our WormAtlas Website (www.wormatlas.org) in an expeditious manner. Website development was one of the original aims of our Center grant, as outlined in our 1997 application. The investigators intend to keep this as a central feature of the grant, but require additional funds in order to hire technical staff, especially an Illustrator, a Data Curator and a Programmer for the Center. The required staffing for Aim 3 greatly exceeds the initial estimates. ? ? The nematode, Caenorhabditis (C.) elegans has been identified as a """"""""high connectivity"""""""" animal for cellular development. This very small animal has been widely adopted for experimental study due to its excellent genetics and accessible cell biology. The complete genome of the worm has been published, and most of the adult anatomy has been reconstructed from electron micrographs (EM) of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most previous EM data are not easily accessed. ? ? The Center for C. elegans Anatomy concentrates on extending EM methods in C. elegans, particularly to: (1) study cellular changes during normal development and in mutant animals; (2) conduct ? EM-immunocytochemical methods to localize antigens at the ultrastructural level; (3) the Center is publishing more detailed information regarding the anatomy of the nematode in the form of annotated electron micrographs, available on the Worm Atlas Website, and on CD-ROMs, by ftp, and eventually in book form; (4) The Anatomy Center houses a large Archive of transmission EM data for the wild type animal and for many mutants. Where necessary, the Center is adding new data to the wild type archive to fill in gaps of knowledge of the adult and larval tissues. This Archive is the source of illustrations for the Atlas project, and is open to visiting scientists for study; (5) the Center provides training in EM methods to visiting students, so that more C. elegans laboratories can conduct their own EM studies; and (6) new and improved methods for EM study of the nematode are under development, and are promulgated to the community. ? ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Resource-Related Research Projects (R24)
Project #
3R24RR012596-06S1
Application #
6685091
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Harding, John D
Project Start
1998-02-01
Project End
2006-02-28
Budget Start
2003-08-01
Budget End
2004-02-29
Support Year
6
Fiscal Year
2003
Total Cost
$232,703
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Neurosciences
Type
Schools of Medicine
DUNS #
071036636
City
Bronx
State
NY
Country
United States
Zip Code
10461
Goupil, Eugénie; Amini, Rana; Hall, David H et al. (2017) Actomyosin contractility regulators stabilize the cytoplasmic bridge between the two primordial germ cells during Caenorhabditis elegans embryogenesis. Mol Biol Cell 28:3789-3800
Gill, Hasreet K; Cohen, Jennifer D; Ayala-Figueroa, Jesus et al. (2016) Integrity of Narrow Epithelial Tubes in the C. elegans Excretory System Requires a Transient Luminal Matrix. PLoS Genet 12:e1006205
Zhang, Jingyan; Li, Xia; Jevince, Angela R et al. (2013) Neuronal target identification requires AHA-1-mediated fine-tuning of Wnt signaling in C. elegans. PLoS Genet 9:e1003618
Hall, David H; Hartwieg, Erika; Nguyen, Ken C Q (2012) Modern electron microscopy methods for C. elegans. Methods Cell Biol 107:93-149
Mancuso, Vincent P; Parry, Jean M; Storer, Luke et al. (2012) Extracellular leucine-rich repeat proteins are required to organize the apical extracellular matrix and maintain epithelial junction integrity in C. elegans. Development 139:979-90
Zhang, Hongjie; Kim, Ahlee; Abraham, Nessy et al. (2012) Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis. Development 139:2071-83
Varshney, Lav R; Chen, Beth L; Paniagua, Eric et al. (2011) Structural properties of the Caenorhabditis elegans neuronal network. PLoS Comput Biol 7:e1001066
O'Hagan, Robert; Piasecki, Brian P; Silva, Malan et al. (2011) The tubulin deglutamylase CCPP-1 regulates the function and stability of sensory cilia in C. elegans. Curr Biol 21:1685-94
Abdus-Saboor, Ishmail; Mancuso, Vincent P; Murray, John I et al. (2011) Notch and Ras promote sequential steps of excretory tube development in C. elegans. Development 138:3545-55
McGee, Matthew D; Weber, Darren; Day, Nicholas et al. (2011) Loss of intestinal nuclei and intestinal integrity in aging C. elegans. Aging Cell 10:699-710

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