Abstract

The applicant proposes to study the structure of a large number of human and animal viruses, virus interactions with receptors, antiviral compounds, and antibodies, as well as assembly intermediates by combination of X-ray crystallography and cryo-electron microscopy. Viruses to be studied include those from the picornavirus family, e.g., rhinovirus, coxsackievirus and poliovirus, and Mengo virus, and from the parvovirus family, e.g. human B19, MVM, porcine, canine and feline parvoviruses. The major focus is to study the interactions of the virus capsid with their receptors and antibodies, and structural changes involved in the process.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI011219-28
Application #
6169352
Study Section
Biophysical Chemistry Study Section (BBCB)
Program Officer
Meegan, James M
Project Start
1976-04-01
Project End
2001-03-31
Budget Start
2000-04-01
Budget End
2001-03-31
Support Year
28
Fiscal Year
2000
Total Cost
$332,900
Indirect Cost

  Institution

Name
Purdue University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907

  Publications

De Castro, Cristina; Klose, Thomas; Speciale, Immacolata et al. (2018) Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate molecular modeling approaches. Proc Natl Acad Sci U S A 115:E44-E52
Zhu, Dongjie; Wang, Xiangxi; Fang, Qianglin et al. (2018) Pushing the resolution limit by correcting the Ewald sphere effect in single-particle Cryo-EM reconstructions. Nat Commun 9:1552
Baggen, Jim; Thibaut, Hendrik Jan; Staring, Jacqueline et al. (2016) Enterovirus D68 receptor requirements unveiled by haploid genetics. Proc Natl Acad Sci U S A 113:1399-404
Liu, Yue; Hill, Marchel G; Klose, Thomas et al. (2016) Atomic structure of a rhinovirus C, a virus species linked to severe childhood asthma. Proc Natl Acad Sci U S A 113:8997-9002
Klose, Thomas; Reteno, Dorine G; Benamar, Samia et al. (2016) Structure of faustovirus, a large dsDNA virus. Proc Natl Acad Sci U S A 113:6206-11
Liu, Yue; Sheng, Ju; Baggen, Jim et al. (2015) Sialic acid-dependent cell entry of human enterovirus D68. Nat Commun 6:8865
Klose, Thomas; Herbst, Dominik A; Zhu, Hanyu et al. (2015) A Mimivirus Enzyme that Participates in Viral Entry. Structure 23:1058-65
Liu, Yue; Sheng, Ju; Fokine, Andrei et al. (2015) Structure and inhibition of EV-D68, a virus that causes respiratory illness in children. Science 347:71-4
Reteno, Dorine Gaëlle; Benamar, Samia; Khalil, Jacques Bou et al. (2015) Faustovirus, an asfarvirus-related new lineage of giant viruses infecting amoebae. J Virol 89:6585-94
Rossmann, Michael G (2014) Serial crystallography using synchrotron radiation. IUCrJ 1:84-6

Showing the most recent 10 out of 73 publications