The objective of this research proposal is to obtain a molecular understanding of the phosphomannosyl targeting system which functions in the delivery of newly synthesized acid hydrolases to lysosomes. Defects in this intracellular protein transport pathway give rise to severe lysosomal storage diseases. A key step in this pathway is the selective phosphorylation of mannose residues on the high mannose glycans of the acid hydrolases by UDP-GlcNAc: lysosomal enzyme N-acetylglucosamine-1- phosphotransferase (Ptase). This transferase is an alpha2 beta2 gamma2 hexameric protein.
Specific Aim 1 is directed toward identifying which subunits of Ptase mediate the recognition of the common protein determinant of acid hydrolases, a process that is essential for the selective phosphorylation of this class of enzymes.
Aim 2 seeks to identify the function of the Man-6-P receptor homology (MRH)-domain of the 3 subunit. We will test the ability of recombinant Ptase with and without its gamma subunit, or with mutations in the MRH domain, to phosphorylate acid hydrolases in in vitro assays and to bind directly to immobilized acid hydrolases using surface plasmon resonance. These studies will be complemented by analyzing the ability of fibroblasts expressing wild type or gamma deficient Ptase to phosphorylate a panel of acid hydrolases transfected into the cells.
Aim 3 is to determine how Ptase is localized to the cis-subcompartment of the Golgi.
Aim 4 is to define the role of the GGA (for Golgi-localized, gamma-ear containing, ARF-binding) proteins in the packaging and transport of the Man-6-P receptors with bound acid hydrolases at the trans-Golgi network. These studies will utilize mice with disruptions of the genes encoding GGA1 and GGA3.
This aim i s designed to establish whether the GGAs are redundant in intact animals and whether there are tissue specific requirements for specific GGAs.

Public Health Relevance

This research is directly relevant to the understanding of two serious lysosomal storage diseases termed MLII and MLIII. Both are caused by mutations in the alpha/beta and gamma genes of Ptase. The work is also relevant to the production of lysosomal enzymes used for enzyme replacement therapy in the treatment of individuals with lysosomal storage diseases such as Fabry and Pompe disease.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Method to Extend Research in Time (MERIT) Award (R37)
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Membrane Biology and Protein Processing (MBPP)
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Snyderwine, Elizabeth G
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Washington University
Internal Medicine/Medicine
Schools of Medicine
Saint Louis
United States
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Liu, Lin; Lee, Wang-Sik; Doray, Balraj et al. (2017) Role of spacer-1 in the maturation and function of GlcNAc-1-phosphotransferase. FEBS Lett 591:47-55
Liu, Lin; Lee, Wang-Sik; Doray, Balraj et al. (2017) Engineering of GlcNAc-1-Phosphotransferase for Production of Highly Phosphorylated Lysosomal Enzymes for Enzyme Replacement Therapy. Mol Ther Methods Clin Dev 5:59-65
van Meel, Eline; Kornfeld, Stuart (2016) Mucolipidosis III GNPTG Missense Mutations Cause Misfolding of the ? Subunit of GlcNAc-1-Phosphotransferase. Hum Mutat 37:623-6
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