Thiamin is an essential component of the human diet with an RDA of 1.2 mg. It is also an important commercial chemical and it is widely used as a food additive and flavoring agent. Annual production, by chemical synthesis, is on the order of 3,300 tons. Thiamin-dependent enzymes play an important role in carbohydrate and branched-chain amino acid metabolism. At this time, our mechanistic understanding of this class of enzymes is sophisticated. In contrast, while thiamin was the first vitamin identified, our understanding of its biosynthesis is still incomplete because the biosynthetic pathway is complex and involves unprecedented reaction chemistry. The long-term goal of our research is the complete mechanistic understanding of the enzymology of thiamin biosynthesis, salvage and catabolism, in all forms of life and the elucidation of connections between thiamin metabolism and other aspects of cellular physiology. In the next grant period, we describe studies designed to elucidate the biosynthesis of the thiamin thiazole and pyrimidine in both bacteria and eukaryotes. These heterocycle forming reactions involve novel biological chemistry. Our strategy will involve a combination of kinetics, modified substrates, protein mutagenesis and structural studies. We will also identify the genes involved in thiamin breakdown and salvage. Bacteria, in the human digestive tract, containing these genes are likely to play an important role in controlling the human thiamin RDA. Finally, we will explore the use of 18F-thiamin as a PET imaging agent, ultimately to determine the global thiamin distrubution in healthy and diseased humans.

Public Health Relevance

Thiamin (vitamin B1) is essential for all forms of life. Our studies will facilitate the commercial production of thiamin by fermentation and have potential applications in the design of new antibiotics. Understanding thiamin catabolism and salvage will enable us to evaluate the importance of these activities in the human digestive tract. 18F-thiamin has potential use, as an imaging agent, in basic human biology and in tumor detection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37DK044083-27
Application #
9265835
Study Section
Special Emphasis Panel (NSS)
Program Officer
Sechi, Salvatore
Project Start
1991-09-15
Project End
2018-04-30
Budget Start
2017-05-01
Budget End
2018-04-30
Support Year
27
Fiscal Year
2017
Total Cost
$332,735
Indirect Cost
$104,834
Name
Texas A&M University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
020271826
City
College Station
State
TX
Country
United States
Zip Code
77845
Wang, Yuanyou; Schnell, Bastien; Baumann, Sascha et al. (2017) Biosynthesis of Branched Alkoxy Groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme. J Am Chem Soc 139:1742-1745
Adak, Sanjoy; Begley, Tadhg P (2017) RutA-Catalyzed Oxidative Cleavage of the Uracil Amide Involves Formation of a Flavin-N5-oxide. Biochemistry 56:3708-3709
Rodrigues, Matthew J; Windeisen, Volker; Zhang, Yang et al. (2017) Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis. Nat Chem Biol 13:290-294
Adak, Sanjoy; Begley, Tadhg P (2016) Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate. J Am Chem Soc 138:6424-6
Eser, Bekir E; Zhang, Xuan; Chanani, Prem K et al. (2016) From Suicide Enzyme to Catalyst: The Iron-Dependent Sulfide Transfer in Methanococcus jannaschii Thiamin Thiazole Biosynthesis. J Am Chem Soc 138:3639-42
Xu, Hui; Chakrabarty, Yindrila; Philmus, Benjamin et al. (2016) Identification of the First Riboflavin Catabolic Gene Cluster Isolated from Microbacterium maritypicum G10. J Biol Chem 291:23506-23515
Nemeria, Natalia S; Shome, Brateen; DeColli, Alicia A et al. (2016) Competence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitamin. Biochemistry 55:1135-48
Jhulki, Isita; Chanani, Prem K; Abdelwahed, Sameh H et al. (2016) A Remarkable Oxidative Cascade That Replaces the Riboflavin C8 Methyl with an Amino Group during Roseoflavin Biosynthesis. J Am Chem Soc 138:8324-7
Zhang, Xuan; Eser, Bekir E; Chanani, Prem K et al. (2016) Structural Basis for Iron-Mediated Sulfur Transfer in Archael and Yeast Thiazole Synthases. Biochemistry 55:1826-38
Fenwick, Michael K; Mehta, Angad P; Zhang, Yang et al. (2015) Non-canonical active site architecture of the radical SAM thiamin pyrimidine synthase. Nat Commun 6:6480

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