The goal of this project is to develop clinical grade serum-free reagents for megakaryocyte proliferation and differentiation. This will permit novel hematopoietic rescue techniques for disorders such as thrombocytopenias. Preliminary data indicate that culture of CD34+ cells in the investigators' serum-free medium outperformed currently available alternatives. The proposed reagents will be optimized with regard to cytokines, culture times and other parameters. In addition to clinical applications, the reagents will enable the standardization of ex vivo protocols in laboratories.

Proposed Commercial Applications

Develop a clinical grade serum-free medium that will support the proliferation/differentiation of megakaryocytes/platelets for reinfusion into patients. Such a technology will be invaluable in fighting diseases/ infections and in coagulation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Small Business Innovation Research Grants (SBIR) - Phase I (R43)
Project #
1R43DK055395-01
Application #
2776475
Study Section
Special Emphasis Panel (ZRG4-HEM-1 (02))
Program Officer
Badman, David G
Project Start
1999-09-01
Project End
2000-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Quality Biological, Inc.
Department
Type
DUNS #
City
Gaithersburg
State
MD
Country
United States
Zip Code
20879