The purpose of this study is to determine if saliva can be used as an adjunct diagnostic fluid for the detection of breast cancer in women. This new diagnostic approach for the detection of breast cancer in women could provide physicians with a safe, simple, highly sensitive, non-invasive, and inexpensive adjunct method for diagnosing women for carcinoma of the breast. These specimens can be obtained easily, readily and repeatedly without discomfort and if predictive for carcinoma of the breast, could possibly decrease the overall morbidity and mortality rates of this lethal and disfiguring disease. Pilot data from the investigators has revealed the presence of a recognized tissue prognostic biomarkers cathepsin-D (CD),epidermal growth factor receptor(EGFR), and cerbB-2(erb),for breast cancer in saliva. Additionally, the markers were elevated in the saliva of women diagnosed with metastatic breast cancer as compared to healthy controls. As a result of the preliminary findings, the aim of this proposal will be to expand the study and compare salivary and serum levels of CD, EGFR, erb, and p53 among patients with breast cancer with axillary lymph node involvement, breast cancer without axillary lymph node involvement, benign breast lesions and a healthy control group in order to determine whether saliva assays would be useful in identifying patients with breast cancer. Saliva and serum CD, EGFR, erb, p53 concentrations will be assayed in specimens obtained from 40 normal healthy women, 40 women diagnosed with breast cancer with lymph node involvement, 40 women diagnosed with breast cancer without lymph node involvement, and 40 women diagnosed with benign breast lesions. EFGR and p53 assays will be performed using ELISA methodology. CD and erb assays will be performed using enzyme-immuno assay (EIA) methodology. The four groups of women will be compared with respect to the saliva and serum CD, EGFR, erb, and p53 levels, the correlation between the saliva and serum CD, EGFR, erb, and p53 concentrations and the predictive.value of saliva CD, EGFR, erb, p53 assays for detecting the presence of malignant breast lesions.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
James A. Shannon Director's Award (R55)
Project #
1R55DE012414-01
Application #
2656027
Study Section
NIDCR Special Grants Review Committee (DSR)
Project Start
1997-09-20
Project End
1999-09-19
Budget Start
1997-09-20
Budget End
1999-09-19
Support Year
1
Fiscal Year
1997
Total Cost
Indirect Cost
Name
University of Mississippi Medical Center
Department
Dentistry
Type
Schools of Dentistry
DUNS #
928824473
City
Jackson
State
MS
Country
United States
Zip Code
39216
Wood, Nelson; Streckfus, Charles F (2015) The Expression of Lung Resistance Protein in Saliva: A Novel Prognostic Indicator Protein for Carcinoma of the Breast. Cancer Invest 33:510-5
Streckfus, C; Bigler, L (2005) The use of soluble, salivary c-erbB-2 for the detection and post-operative follow-up of breast cancer in women: the results of a five-year translational research study. Adv Dent Res 18:17-24
Streckfus, Charles F; Bigler, Lenora; Dellinger, Tracy et al. (2004) The expression of the c-erbB-2 receptor protein in glandular salivary secretions. J Oral Pathol Med 33:595-600
Brinkley, Jack; Copeland, Lynn; Streckfus, Charles et al. (2003) Sustained delivery of Her-2/neu antibody by TCPL delivery device using adult male rats as a model. Biomed Sci Instrum 39:324-8
Bigler, Lenora R; Streckfus, Charles F; Copeland, Lynn et al. (2002) The potential use of saliva to detect recurrence of disease in women with breast carcinoma. J Oral Pathol Med 31:421-31
Streckfus, C; Bigler, L; Dellinger, T et al. (2001) Reliability assessment of soluble c-erbB-2 concentrations in the saliva of healthy women and men. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:174-9
Streckfus, C; Bigler, L; Dellinger, T et al. (2000) The presence of soluble c-erbB-2 in saliva and serum among women with breast carcinoma: a preliminary study. Clin Cancer Res 6:2363-70
Streckfus, C; Bigler, L; Tucci, M et al. (2000) A preliminary study of CA15-3, c-erbB-2, epidermal growth factor receptor, cathepsin-D, and p53 in saliva among women with breast carcinoma. Cancer Invest 18:101-9
Streckfus, C; Bigler, L; Dellinger, T et al. (1999) CA 15-3 and c-erbB-2 presence in the saliva of women. Clin Oral Investig 3:138-43
McIntyre, R; Bigler, L; Dellinger, T et al. (1999) Oral contraceptive usage and the expression of CA 15-3 and c-erbB-2 in the saliva of healthy women. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 88:687-90