Abstract

This research proposal focuses on the molecular details of how the E. coli rho protein determines the location for the dissociation of the ternary transcription complex at specific sites on the DNA template. A model has been previously suggested that postulates that the interaction of rho protein with large unstructured regions of the transcript activates the ATPase-dependent rho-catalyzed transcript release reaction. RNA polymerase pause sites kinetically determine the position on the template where the release reaction is most likely to take place. This model will be used to design synthetic rho-dependent termination sites that will be inserted into plasmid expression vectors. The function of these sites will be evaluated in purified in vitro transcription reactions as well as in vivo.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Minority Biomedical Research Support - MBRS (S06)
Project #
5S06GM008139-16
Application #
3877448
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
University of New Mexico
Department
Type
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131

  Publications

Bharadwaj, D; Mold, C; Markham, E et al. (2001) Serum amyloid P component binds to Fc gamma receptors and opsonizes particles for phagocytosis. J Immunol 166:6735-41
Romero, I R; Morris, C; Rodriguez, M et al. (1998) Inflammatory potential of C-reactive protein complexes compared to immune complexes. Clin Immunol Immunopathol 87:155-62
Tetzloff, S U; Bizzozero, O A (1998) Palmitoylation of proteolipid protein from rat brain myelin using endogenously generated 18O-fatty acids. J Biol Chem 273:279-85
Sanchez, P; Tetzloff, S U; Bizzozero, O A (1998) Veratridine-induced depolarization reduces the palmitoylation of brain and myelin glycerolipids. J Neurochem 70:1448-57
Bryant, J E; Hutchings, K G; Moyzis, R K et al. (1997) Measurement of telomeric DNA content in human tissues. Biotechniques 23:476-8, 480, 482, passim
Melendez, R F; Bizzozero, O A (1996) Palmitoylation of myelin P0 protein is independent of its synthesis and parallels that of phospholipids. J Peripher Nerv Syst 1:34-41
Mold, C; Gurule, C; Otero, D et al. (1996) Complement-dependent binding of C-reactive protein complexes to human erythrocyte CR1. Clin Immunol Immunopathol 81:153-60
Chapin, J E; Davis, L E; Kornfeld, M et al. (1995) Neurologic manifestations of intravascular lymphomatosis. Acta Neurol Scand 91:494-9
Smith, J P; Hicks, P S; Ortiz, L R et al. (1995) Quantitative measurement of muscle strength in the mouse. J Neurosci Methods 62:15-9
Varela, M F; Sansom, C E; Griffith, J K (1995) Mutational analysis and molecular modelling of an amino acid sequence motif conserved in antiporters but not symporters in a transporter superfamily. Mol Membr Biol 12:313-9

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