Funding is requested for replacing the console of a high-resolution 600 MHz NMR spectrometer at Northwestern University's Biological NMR Center, a unique resource providing structural and chemical biologists access to high-field instrumentation within a single facility for inter- disciplinary research. Since 1990, with support from NIH and NSF, Northwestern researchers have established and maintained an outstanding resource for macromolecular NMR that has had a significant positive impact on the research programs of multiple investigators both within the college and the university at large. The 600 MHz spectrometer, which is the one and only NMR instrument in the facility, is heavily and continuously used. However, the reliability of this instrument has decreased lately because of a range of problems (including a most persistent FIFO underflow error problem) associated with an aging console, which was last replaced in 1999. The acquisition of a new console along with a replacement compressor (aka cold pack) for the cold probe would cater to the heavy demand for NMR time by a user base that comprises seven well-funded major research groups working on a variety of basic and clinical problems in biology or at the chemistry-biology interface. The research topics being studied include structure-function analyses of transcription factors, structural and functional genomics of infectious agents, electron transfer via protein-protein interactions, structural characterization of natural products, structural biology of kinesin regulation, design and development of novel inhibitors and contrast agents for magnetic resonance imaging and diagnostic applications, and novel synthetic chemistry approaches for medicinal applications. The projects have strong structural and/or functional foci and all of them will benefit from the added sensitivity (mainly from improvements in hardware that have occurred in the past decade), but most significantly, added reliability afforded by a new console and cold probe compressor. The proposed instrument will have all the capabilities to perform any contemporary biomolecular NMR pulse sequence experiment. Since the magnet and cold probe (other than the compressor) have been determined to be in good shape, there are no plans to replace either of them. The new console will have four channels with the first channel for 1H excitation, decoupling and detection, a second channel for excitation, decoupling and broadband detection of biologically-relevant heteronuclei, and a dual channel for excitation and decoupling of biologically-relevant heteronuclei, and pulsed field gradient accessories.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10OD012016-01
Application #
8247293
Study Section
Special Emphasis Panel (ZRG1-BCMB-U (30))
Program Officer
Levy, Abraham
Project Start
2012-04-01
Project End
2013-03-31
Budget Start
2012-04-01
Budget End
2013-03-31
Support Year
1
Fiscal Year
2012
Total Cost
$267,428
Indirect Cost
Name
Northwestern University at Chicago
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
160079455
City
Evanston
State
IL
Country
United States
Zip Code
60201
Daffern, Nicolas; Chen, Zhonglei; Zhang, Yongbo et al. (2018) Solution Nuclear Magnetic Resonance Studies of the Ligand-Binding Domain of an Orphan Nuclear Receptor Reveal a Dynamic Helix in the Ligand-Binding Pocket. Biochemistry 57:1977-1986
Li, Zhanyong; Peters, Aaron W; Liu, Jian et al. (2017) Size Effect of the Active Sites in UiO-66-Supported Nickel Catalysts Synthesized via Atomic Layer Deposition for Ethylene Hydrogenation. Inorg Chem Front 4:820-824
Calzada, Raul; Thompson, Christopher M; Westmoreland, Dana E et al. (2016) Organic-to-Aqueous Phase Transfer of Cadmium Chalcogenide Quantum Dots using a Sulfur-Free Ligand for Enhanced Photoluminescence and Oxidative Stability. Chem Mater 28:6716-6723
Henke, Matthew T; Soukup, Alexandra A; Goering, Anthony W et al. (2016) New Aspercryptins, Lipopeptide Natural Products, Revealed by HDAC Inhibition in Aspergillus nidulans. ACS Chem Biol 11:2117-23
McClure, Ryan A; Goering, Anthony W; Ju, Kou-San et al. (2016) Elucidating the Rimosamide-Detoxin Natural Product Families and Their Biosynthesis Using Metabolite/Gene Cluster Correlations. ACS Chem Biol 11:3452-3460
Li, Zhanyong; Schweitzer, Neil M; League, Aaron B et al. (2016) Sintering-Resistant Single-Site Nickel Catalyst Supported by Metal-Organic Framework. J Am Chem Soc 138:1977-82
Goering, Anthony W; McClure, Ryan A; Doroghazi, James R et al. (2016) Metabologenomics: Correlation of Microbial Gene Clusters with Metabolites Drives Discovery of a Nonribosomal Peptide with an Unusual Amino Acid Monomer. ACS Cent Sci 2:99-108
Xie, Tao; Zmyslowski, Adam M; Zhang, Yongbo et al. (2015) Structural Basis for Multi-specificity of MRG Domains. Structure 23:1049-57
Clark, Michael David; Zhang, Yongbo; Radhakrishnan, Ishwar (2015) Solution NMR Studies of an Alternative Mode of Sin3 Engagement by the Sds3 Subunit in the Histone Deacetylase-Associated Sin3L/Rpd3L Corepressor Complex. J Mol Biol 427:3817-23
Clark, Michael D; Marcum, Ryan; Graveline, Richard et al. (2015) Structural insights into the assembly of the histone deacetylase-associated Sin3L/Rpd3L corepressor complex. Proc Natl Acad Sci U S A 112:E3669-78

Showing the most recent 10 out of 11 publications