Abstract

The NYU Mass Spectrometry Core Facility for Neuroscience, the 100 Women in Hedge Funds Clinical Proteomics Facility and the NYU Protein Analysis Facility within the Kimmel Center for Molecular Biomedicine at the Skirball Institute at the NYU School of Medicine are dedicated to ensuring that biomedical scientists have access to cutting edge mass spectrometry technology to enable their research. We are requesting funds for a Thermo Electron LTQ-Orbitrap Velos mass spectrometer to make possible the accurate and sensitive detection, identification, characterization and relative quantitation of proteins and peptides involved in many important diseases for 11 NIH-funded major users and 5 minor users. In addition, the requested instrument would enable stable isotope labeling (SILAC) experiments for the study of intracellular signal transduction in neurons and other cells. These experiments would greatly increase our understanding of the causes and mechanisms of a number of neurodegenerative, developmental, infectious, and neuromuscular diseases and cancer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Shared Instrumentation Grants (S10)
Project #
1S10RR027990-01A1
Application #
8052013
Study Section
Special Emphasis Panel (ZRG1-BCMB-M (30))
Program Officer
Levy, Abraham
Project Start
2011-04-01
Project End
2012-03-31
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
1
Fiscal Year
2011
Total Cost
$600,000
Indirect Cost

  Institution

Name
New York University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016

  Publications

Deng, Jingjing; Erdjument-Bromage, Hediye; Neubert, Thomas A (2018) Quantitative Comparison of Proteomes Using SILAC. Curr Protoc Protein Sci :e74
Wang, Zheng; Gearhart, Micah D; Lee, Yu-Wei et al. (2018) A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs. Cell Stem Cell 22:235-251.e9
Hernandez, Charles; Wang, Zheng; Ramazanov, Bulat et al. (2018) Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency. Cell Stem Cell 23:396-411.e8
Cabrera, Erwin; Mathews, Paul; Mezhericher, Emiliya et al. (2018) A? truncated species: Implications for brain clearance mechanisms and amyloid plaque deposition. Biochim Biophys Acta Mol Basis Dis 1864:208-225
Erdjument-Bromage, Hediye; Huang, Fang-Ke; Neubert, Thomas A (2018) Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS). Methods Mol Biol 1741:135-149
Choi, Meena; Eren-Dogu, Zeynep F; Colangelo, Christopher et al. (2017) ABRF Proteome Informatics Research Group (iPRG) 2015 Study: Detection of Differentially Abundant Proteins in Label-Free Quantitative LC-MS/MS Experiments. J Proteome Res 16:945-957
Gauthier, Sébastien A; Pérez-González, Rocío; Sharma, Ajay et al. (2017) Enhanced exosome secretion in Down syndrome brain - a protective mechanism to alleviate neuronal endosomal abnormalities. Acta Neuropathol Commun 5:65
Snuderl, Matija; Zhang, Guoan; Wu, Pamela et al. (2017) Endothelium-Independent Primitive Myxoid Vascularization Creates Invertebrate-Like Channels to Maintain Blood Supply in Optic Gliomas. Am J Pathol 187:1867-1878
Kang, Min-Ji; Vasudevan, Deepika; Kang, Kwonyoon et al. (2017) 4E-BP is a target of the GCN2-ATF4 pathway during Drosophila development and aging. J Cell Biol 216:115-129
Huang, Fang-Ke; Zhang, Guoan; Lawlor, Kevin et al. (2017) Deep Coverage of Global Protein Expression and Phosphorylation in Breast Tumor Cell Lines Using TMT 10-plex Isobaric Labeling. J Proteome Res 16:1121-1132

Showing the most recent 10 out of 25 publications