Developmental Virology Research

Chen, Irvin S.Y.

Abstract

This Virology Developmental Research component is designed to utilize and develop virological markers to investigate virus replication at the levels of DNA synthesis (reverse transcription) and gene expression. In addition, these various markers of viral replication would be utilized to investigate viral resistance profiles of individuals infected with human immunodeficiency virus type 1 (HIV-1). The UCLA patient population of the ACTG is ideal for this purpose. 1. To determine if a correlation exists between advanced clinical disease and/or virus expression and expression of viral regulatory genes. 2. To determine the viral resistance profiles of individuals infected with HIV-1 utilizing an in vitro assay on once-passaged isolates or directly on virus from plasma and peripheral blood mononuclear cells (PBMC) (ex vivo). 3. To develop novel assays utilizing PCR to directly monitor the extent of HIV-1 reverse transcription following infection. These assays will be used to monitor the degree of resistance to reverse transcriptase inhibitors of virus from ACTG patients.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research Project--Cooperative Agreements (U01)
• Project #: 5U01AI027660-08
• Application #: 3746917
• Support Year: 8
• Fiscal Year: 1994

Institution

• Name: University of California Los Angeles
• DUNS #: 119132785
• City: Los Angeles
• State: CA
• Country: United States
• Zip Code: 90095

Publications

Kempen, John H; Sugar, Elizabeth A; Varma, Rohit et al. (2014) Risk of cataract among subjects with acquired immune deficiency syndrome free of ocular opportunistic infections. Ophthalmology 121:2317-24

Kozak, Igor; Vaidya, Vijay; Van Natta, Mark L et al. (2014) The prevalence and incidence of epiretinal membranes in eyes with inactive extramacular CMV retinitis. Invest Ophthalmol Vis Sci 55:4304-12

Jabs, Douglas A; Ahuja, Alka; Van Natta, Mark et al. (2013) Comparison of treatment regimens for cytomegalovirus retinitis in patients with AIDS in the era of highly active antiretroviral therapy. Ophthalmology 120:1262-70

Wohl, David A; Kendall, Michelle A; Feinberg, Judith et al. (2013) The clinical impact of continuing to prescribe antiretroviral therapy in patients with advanced AIDS who manifest no virologic or immunologic benefit. PLoS One 8:e78676

Gangaputra, Sapna; Drye, Lea; Vaidya, Vijay et al. (2013) Non-cytomegalovirus ocular opportunistic infections in patients with acquired immunodeficiency syndrome. Am J Ophthalmol 155:206-212.e5

Ribaudo, Heather J; Smith, Kimberly Y; Robbins, Gregory K et al. (2013) Racial differences in response to antiretroviral therapy for HIV infection: an AIDS clinical trials group (ACTG) study analysis. Clin Infect Dis 57:1607-17

Kempen, John H; Sugar, Elizabeth A; Lyon, Alice T et al. (2012) Risk of cataract in persons with cytomegalovirus retinitis and the acquired immune deficiency syndrome. Ophthalmology 119:2343-50

Gangaputra, Sapna; Kalyani, Partho S; Fawzi, Amani A et al. (2012) Retinal vessel caliber among people with acquired immunodeficiency syndrome: relationships with disease-associated factors and mortality. Am J Ophthalmol 153:434-444.e1

Kalyani, Partho S; Fawzi, Amani A; Gangaputra, Sapna et al. (2012) Retinal vessel caliber among people with acquired immunodeficiency syndrome: relationships with visual function. Am J Ophthalmol 153:428-433.e1

Kalyani, Partho S; Holland, Gary N; Fawzi, Amani A et al. (2012) Association between retinal nerve fiber layer thickness and abnormalities of vision in people with human immunodeficiency virus infection. Am J Ophthalmol 153:734-42, 742.e1