Some estimate of radiation dose is critical in the initial stages of triage after a terrorist nuclear event; this proposal will focus on the demonstration of a fast, portable, non-invasive instrument that will measure radiation dose to intact teeth by optical stimulation of luminescence (OSL). Our goal is to develop the necessary information on teeth: (a) to enable optimization of OSL measurement parameters; (b) to determine the range of person-to-person variability of OSL characteristics to gamma radiation, and (c) to demonstrate portable instrumentation capable of making measurements on teeth in the critical zone of 0.5 to 10 Gy. This demonstration will facilitate commercialization of an instrument that will enable medical personnel, in a radiation emergency situation, to distinguish people with low or no exposures from those requiring medical attention. There is currently no rapid method to make this identification. 1. Determine the uncertainty in whole body dose based on a unit dose to a molar, and for the different radiation types that are expected in RDDs. This set of calculations will provide the translation between OSL signals from the tooth and radiation impact to the individual. 2. Optimize signal-to-noise in OSL of irradiated porcine molars. At each of our three laboratories, we will use a variety of techniques, including the investigation of different excitation wavelengths, stimulation modes, and minor surface preparations on fresh porcine molars to optimize signal recovery. We will evaluate conditions of signal bleaching and determine the similarity of human teeth to porcine teeth. 3. Modify equipment at Oklahoma State University to optimize signal recovery at the tooth-fiber optic interface and optimize other components based on Aim # 2. 4. Characterize the age, gender and racial differences in OSL of teeth to radiation. Perform OSL dose response measurements in teeth.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19AI067798-01
Application #
7052917
Study Section
Special Emphasis Panel (ZCA1-SRRB-E (O1))
Project Start
2005-09-01
Project End
2010-07-31
Budget Start
2005-09-01
Budget End
2006-07-31
Support Year
1
Fiscal Year
2005
Total Cost
$265,510
Indirect Cost
Name
Duke University
Department
Type
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Cline, John Mark; Dugan, Greg; Bourland, John Daniel et al. (2018) Post-Irradiation Treatment with a Superoxide Dismutase Mimic, MnTnHex-2-PyP5+, Mitigates Radiation Injury in the Lungs of Non-Human Primates after Whole-Thorax Exposure to Ionizing Radiation. Antioxidants (Basel) 7:
Farris, Michael; McTyre, Emory R; Okoukoni, Catherine et al. (2018) Cortical Thinning and Structural Bone Changes in Non-Human Primates after Single-Fraction Whole-Chest Irradiation. Radiat Res 190:63-71
Naqvi, Ibtehaj; Gunaratne, Ruwan; McDade, Jessica E et al. (2018) Polymer-Mediated Inhibition of Pro-invasive Nucleic Acid DAMPs and Microvesicles Limits Pancreatic Cancer Metastasis. Mol Ther 26:1020-1031
Ghandhi, Shanaz A; Turner, Helen C; Shuryak, Igor et al. (2018) Whole thorax irradiation of non-human primates induces persistent nuclear damage and gene expression changes in peripheral blood cells. PLoS One 13:e0191402
Castle, Katherine D; Daniel, Andrea R; Moding, Everett J et al. (2018) Mice Lacking RIP3 Kinase are not Protected from Acute Radiation Syndrome. Radiat Res 189:627-633
Fanning, K M; Pfisterer, B; Davis, A T et al. (2017) Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling. Am J Physiol Regul Integr Comp Physiol 313:R290-R297
Swanson, Karen V; Junkins, Robert D; Kurkjian, Cathryn J et al. (2017) A noncanonical function of cGAMP in inflammasome priming and activation. J Exp Med 214:3611-3626
Kurkjian, Cathryn J; Guo, Hao; Montgomery, Nathan D et al. (2017) The Toll-Like Receptor 2/6 Agonist, FSL-1 Lipopeptide, Therapeutically Mitigates Acute Radiation Syndrome. Sci Rep 7:17355
Racioppi, Luigi; Lento, William; Huang, Wei et al. (2017) Calcium/calmodulin-dependent kinase kinase 2 regulates hematopoietic stem and progenitor cell regeneration. Cell Death Dis 8:e3076
Himburg, Heather A; Doan, Phuong L; Quarmyne, Mamle et al. (2017) Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med 23:91-99

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