The liver evolves mechanism(s) to dampen and possibly suppress the host immunity to nonpathogenic food antigens. As a result, liver tropic viruses such as HCV establish the persistent infection in the liver due impaired CD8+ T cell responses. NK cells are highly enriched in the liver and act as innate defense to viral infection. Following conjugation between NK cells and target cells, NK cells are activated and express NK activating/inhibitory receptors to trigger NK activity. Signaling through intimate interaction of NK activation/inhibitory receptors is critical for controlling NK activities. NK cells have been reported to play a pivotal role in linking innate immunity to adaptive immunity, particularly regulation of intrahepatic CDS* T cell responses during viral infection. We have recently established a murine model system via two different routes (i.e. IV vs SubQ) of adenovirus infection, which is an important system to dissect the mechanism for regulation of intrahepatic CD8+ T cell responses. IV inoculation predominantly delivers viral antigen to the liver while SubQ injection expresses antigen into the lymph nodes. By employing this murine model, we found that different route of adenovirus infection influence the magnitude and effector function of CDS* T cell responses. The impaired CDS* T cell responses were found in the liver of mice following IV infection. In contrast, mice infected SubQ adenovirus infection generates the effective CDS* T cell responses. In addition, NKG2A*NK cells and IL-10-producing DC are increased in the liver from IV Ad-LacZ infected mice. Intriguingly, the in vivo depletion of NK cells reversed the dysregulation of antiviral CDS* T cell responses. These results, coupled with evidence that the inhibitory function of liver NK cells, suggest a potential mechanism for NK cell-mediated in inhibition of CDS* T cell effector function via NK-DC cross-talk. In this proposal, we will characterize the effect of NK cells on affecting CDS* T cell responses and determine the mechanism for NK-mediated impairment of CDS* T cell effector function via altering DC activation. These studies may provide a useful information regarding the regulatory role of NK cell dysregulation in generation of effective CDS* T cell responses as well as new insights for immunotherapeutic strategies against viral infection.

Public Health Relevance

Viral infection in the liver often establishes persistent infection. This could be due to the failure of generating effective T cell responses to liver infection in the host. The main goal of Project 2 is to determine how liver viral infection suppresses the host T cell immunity and to identify a potential mechanism of regulation of T cell immunity via altered innate immunity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI083024-02
Application #
8096714
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
2
Fiscal Year
2010
Total Cost
$258,427
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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Tosello-Trampont, Annie-Carole; Krueger, Peter; Narayanan, Sowmya et al. (2016) NKp46(+) natural killer cells attenuate metabolism-induced hepatic fibrosis by regulating macrophage activation in mice. Hepatology 63:799-812
DeBerge, Matthew P; Ely, Kenneth H; Wright, Peter F et al. (2015) Shedding of TNF receptor 2 by effector CD8? T cells by ADAM17 is important for regulating TNF-? availability during influenza infection. J Leukoc Biol 98:423-34
Krueger, Peter D; Kim, Taeg S; Sung, Sun-Sang J et al. (2015) Liver-resident CD103+ dendritic cells prime antiviral CD8+ T cells in situ. J Immunol 194:3213-22

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