An Immunologic Reagent Resource Core will be established in support of the three research projects to provide critical reagents to track the changes in immune phenotype in response to vaccination and infection. Antibodies and tetramers are unique reagents and have been proven to be powerful tools to facilitate innovative research in many fields of biomedical science. We have been successful in our effort to create and characterize a panel of monoclonal antibodies and Ig related fusion proteins to soluble and cell surface proteins using a variety of techniques. Specifically, the Resource will produce monoclonal antibodies from hybridomas that are already available in the core or obtained from the ATCC or individual investigators. The antibodies will be conjugated to the appropriate fluorochromes as specified in the research projects. In addition, the antibody core will establish a quality control system for production and testing of specificity, quality, and concentration of reagents before providing them to the individual projects. In collaboration with Dr. Bill Kwok, the core will also generate a series of class I and Class II tetramers, which will be used to track antigen-specific T cell response following infection or vaccination. This large-scale, coordinated effort will result in high quality reagents for the projects at a substantially reduced overall cost.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI089992-04
Application #
8667884
Study Section
Special Emphasis Panel (ZAI1-QV-I (M2))
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
4
Fiscal Year
2013
Total Cost
$49,643
Indirect Cost
$14,931
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Murray, Kristy O; Nolan, Melissa S; Ronca, Shannon E et al. (2018) The Neurocognitive and MRI Outcomes of West Nile Virus Infection: Preliminary Analysis Using an External Control Group. Front Neurol 9:111
Molony, Ryan D; Malawista, Anna; Montgomery, Ruth R (2018) Reduced dynamic range of antiviral innate immune responses in aging. Exp Gerontol 107:130-135
Martin-Gayo, Enrique; Cole, Michael B; Kolb, Kellie E et al. (2018) A Reproducibility-Based Computational Framework Identifies an Inducible, Enhanced Antiviral State in Dendritic Cells from HIV-1 Elite Controllers. Genome Biol 19:10
Wang, Xiaomei; Malawista, Anna; Qian, Feng et al. (2018) Age-related changes in expression and signaling of TAM receptor inflammatory regulators in monocytes. Oncotarget 9:9572-9580
Cahill, Megan E; Conley, Samantha; DeWan, Andrew T et al. (2018) Identification of genetic variants associated with dengue or West Nile virus disease: a systematic review and meta-analysis. BMC Infect Dis 18:282
van Dijk, David; Sharma, Roshan; Nainys, Juozas et al. (2018) Recovering Gene Interactions from Single-Cell Data Using Data Diffusion. Cell 174:716-729.e27
Ordovas-Montanes, Jose; Dwyer, Daniel F; Nyquist, Sarah K et al. (2018) Allergic inflammatory memory in human respiratory epithelial progenitor cells. Nature 560:649-654
Mead, Benjamin E; Ordovas-Montanes, Jose; Braun, Alexandra P et al. (2018) Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types. BMC Biol 16:62
Avey, Stefan; Mohanty, Subhasis; Wilson, Jean et al. (2017) Multiple network-constrained regressions expand insights into influenza vaccination responses. Bioinformatics 33:i208-i216
Cahill, Megan E; Yao, Yi; Nock, David et al. (2017) West Nile Virus Seroprevalence, Connecticut, USA, 2000-2014. Emerg Infect Dis 23:708-710

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