Abstract

The Protein Expression Core will provide monoclonal antibodies (mAb) and recombinant chimeric Fc fusion proteins to support all programs of this NCDDG. The Core will provide purified and characterized proteins, suitable for both the in vitro and in vivo analyses. This centralized core will allow for comparative studies among this NCDDG since these agents will be used from equivalent, quality control lots of production. The in vivo feasibility and optimization data generated using the Core-generated reagents will be suitable for including in investigator-initiated INDs submitted to support the clinical trials that arise from this NCDDG program. Each agent will be expressed, purified, and subjected to quality control studies and each agent will be produced under standard operating procedures, tested, and distributed to investigators in the program as needed. Specifically, we are going to express and purify recombinant Fc-fusion proteins and mAbs, perform quality control assays to demonstrate and quantitate bioactivity, perform sterility and endotoxin testing, and and distribute to all investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19CA113341-05
Application #
7816978
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2009-05-01
Budget End
2010-04-30
Support Year
5
Fiscal Year
2009
Total Cost
$76,359
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Keenan, Bridget P; Saenger, Yvonne; Kafrouni, Michel I et al. (2014) A Listeria vaccine and depletion of T-regulatory cells activate immunity against early stage pancreatic intraepithelial neoplasms and prolong survival of mice. Gastroenterology 146:1784-94.e6
Yao, Sheng; Zhu, Yuwen; Chen, Lieping (2013) Advances in targeting cell surface signalling molecules for immune modulation. Nat Rev Drug Discov 12:130-46
Zhu, Yuwen; Yao, Sheng; Iliopoulou, Bettina P et al. (2013) B7-H5 costimulates human T cells via CD28H. Nat Commun 4:2043
Yao, Sheng; Zhu, Yuwen; Zhu, Gefeng et al. (2011) B7-h2 is a costimulatory ligand for CD28 in human. Immunity 34:729-40
Uram, Jennifer N; Black, Chelsea M; Flynn, Emilee et al. (2011) Nondominant CD8 T cells are active players in the vaccine-induced antitumor immune response. J Immunol 186:3847-57
Pfannenstiel, Lukas W; Lam, Samuel S K; Emens, Leisha A et al. (2010) Paclitaxel enhances early dendritic cell maturation and function through TLR4 signaling in mice. Cell Immunol 263:79-87
Zhang, Yu-Qian; Tsai, Ya-Chea; Monie, Archana et al. (2010) Enhancing the therapeutic effect against ovarian cancer through a combination of viral oncolysis and antigen-specific immunotherapy. Mol Ther 18:692-9
Mao, Chih-Ping; Wu, T-C (2010) Inhibitory RNA molecules in immunotherapy for cancer. Methods Mol Biol 623:325-39
Kim, Daejin; Hoory, Talia; Monie, Archana et al. (2010) Delivery of chemotherapeutic agents using drug-loaded irradiated tumor cells to treat murine ovarian tumors. J Biomed Sci 17:61
Talay, Oezcan; Shen, Ching-Hung; Chen, Lieping et al. (2009) B7-H1 (PD-L1) on T cells is required for T-cell-mediated conditioning of dendritic cell maturation. Proc Natl Acad Sci U S A 106:2741-6

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