Abstract

The selection of model ENMs used for the RESAC Center projects is largely driven by the over-riding hypothesis of the RESAC toxicological projects (Projects 1 and 2), that (i) the cellular, molecular and immune reactivities in response to ENMs depend on the specific physicochemical properties of the ENMs;(ii) the physicochemical properties of the ENMs deposited in the lungs critically depend on their interaction with lung lining fluid, which in turn impacts on ENM reactivity with macrophages and epithelial cells;(iii) in turn, this determines their entry, localization, biological activity and fate in the lung. The selection is also based on potentials for exposure and health risks. We plan to study two types of commercially widely-used ENMs: silver and carbon nanomaterials. Their specific physicochemical properties are summarized in Table SCI. Our selection of these two ENMs allows us to compare biological effects in two systems of great technological importance. By preparing well-controlled ENMs, standardized by particle size and aspect ratio, we will be able to move beyond the particular behaviour of a single chemically homogenous system (whether silver or carbon), in order to isolate, more fundamentally, the effects of geometry and chemistry.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program--Cooperative Agreements (U19)
Project #
1U19ES019536-01
Application #
8068440
Study Section
Special Emphasis Panel (ZES1-SET-V (03))
Project Start
2010-09-28
Project End
2015-04-30
Budget Start
2010-09-28
Budget End
2011-04-30
Support Year
1
Fiscal Year
2010
Total Cost
$86,400
Indirect Cost

  Institution

Name
University of Southern California
Department
Type
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089

  Publications

Botelho, Danielle; Leo, Bey F; Massa, Christopher et al. (2018) Exposure to Silver Nanospheres Leads to Altered Respiratory Mechanics and Delayed Immune Response in an in Vivo Murine Model. Front Pharmacol 9:213
Chung, Kian Fan; Seiffert, Joanna; Chen, Shu et al. (2017) Inactivation, Clearance, and Functional Effects of Lung-Instilled Short and Long Silver Nanowires in Rats. ACS Nano 11:2652-2664
Theodorou, Ioannis G; Ruenraroengsak, Pakatip; Gow, Andrew et al. (2016) Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells. Nanotoxicology 10:1351-62
Seiffert, Joanna; Buckley, Alison; Leo, Bey et al. (2016) Pulmonary effects of inhalation of spark-generated silver nanoparticles in Brown-Norway and Sprague-Dawley rats. Respir Res 17:85
Ruenraroengsak, Pakatip; Chen, Shu; Hu, Sheng et al. (2016) Translocation of Functionalized Multi-Walled Carbon Nanotubes across Human Pulmonary Alveolar Epithelium: Dominant Role of Epithelial Type 1 Cells. ACS Nano 10:5070-85
Chen, S; Goode, A E; Skepper, J N et al. (2016) Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments. J Microsc 261:157-66
Botelho, Danielle J; Leo, Bey Fen; Massa, Christopher B et al. (2016) Low-dose AgNPs reduce lung mechanical function and innate immune defense in the absence of cellular toxicity. Nanotoxicology 10:118-27
Govada, Lata; Leese, Hannah S; Saridakis, Emmanuel et al. (2016) Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals. Sci Rep 6:20053
Seiffert, Joanna; Hussain, Farhana; Wiegman, Coen et al. (2015) Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726
Sarkar, Srijata; Leo, Bey Fen; Carranza, Claudia et al. (2015) Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification. PLoS One 10:e0143077

Showing the most recent 10 out of 29 publications