Precise metabolic phenotyping depends significantly on the health status of animal subjects. Stable housing and husbandry and microbiological testing minimize the influence of exogenous factors that could impact phenotyping results. The rigors inherent to quarantine and testing mice to ensure acceptable health status must be balanced with the need to expedite phenotyping of newly arrived mice. The Animal Core will provide state-of-the-art husbandry, health care, quarantine, testing, and procedure space for assessing mice submitted to the Mouse Metabolic Phenotyping Center (MMPC). The Animal Care Core has implemented an expedited quarantine program that has been effect for the past 9 years. This has successfully decreased the quarantine time and allowed phenotyping to begin while animals are within quarantine, allowing animals to be studied at younger ages and minimizing costs associated with the phenotyping process. The Core will: 1) import mice for metabolic phenotyping, 2) determine the microbiological profile of each imported cohort and provide the Center Director with interpretation and advice about the results, 3) provide stable, biocontainment housing, husbandry and health care for mice submitted to the Center, 4) provide a dedicated, biosecure suite of room for quarantine, housing and on-site phenotyping procedures, 5) perform morphological phenotyping as requested, 6) provide to Center clients results and advice on the health status of submitted mice. The facilities include 600 ft^ of dedicated biocontainment housing and procedure space equipped with 160 cage ventilated rack, an NMR analyzer, and 8 metabolic (calorimetry) cages. The core is expected to import 802 +/- 215 mice per year, based on core volume during the previous 5 years.

Public Health Relevance

It Is anticipated that the results obtained from characterizing these mice at the Yale MMPC will provide important new insights into the pathogenesis and complications relating to diabetes and obesity in humans.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Projects--Cooperative Agreements (U24)
Project #
2U24DK059635-06
Application #
8204227
Study Section
Special Emphasis Panel (ZDK1-GRB-S (M1))
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2011
Total Cost
$152,558
Indirect Cost
Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Qiu, Yang; Perry, Rachel J; Camporez, João-Paulo G et al. (2018) In vivo studies on the mechanism of methylene cyclopropyl acetic acid and methylene cyclopropyl glycine-induced hypoglycemia. Biochem J 475:1063-1074
Budatha, Madhusudhan; Zhang, Jiasheng; Zhuang, Zhen W et al. (2018) Inhibiting Integrin ?5 Cytoplasmic Domain Signaling Reduces Atherosclerosis and Promotes Arteriogenesis. J Am Heart Assoc 7:
Jelenik, Tomas; Flögel, Ulrich; Álvarez-Hernández, Elisa et al. (2018) Insulin Resistance and Vulnerability to Cardiac Ischemia. Diabetes 67:2695-2702
Corbit, Kevin C; Camporez, João Paulo G; Edmunds, Lia R et al. (2018) Adipocyte JAK2 Regulates Hepatic Insulin Sensitivity Independently of Body Composition, Liver Lipid Content, and Hepatic Insulin Signaling. Diabetes 67:208-221
Perry, Rachel J; Peng, Liang; Cline, Gary W et al. (2018) Mechanisms by which a Very-Low-Calorie Diet Reverses Hyperglycemia in a Rat Model of Type 2 Diabetes. Cell Metab 27:210-217.e3
Majtan, Tomas; Jones Jr, Wendell; Krijt, Jakub et al. (2018) Enzyme Replacement Therapy Ameliorates Multiple Symptoms of Murine Homocystinuria. Mol Ther 26:834-844
Perry, Rachel J; Wang, Yongliang; Cline, Gary W et al. (2018) Leptin Mediates a Glucose-Fatty Acid Cycle to Maintain Glucose Homeostasis in Starvation. Cell 172:234-248.e17
Lawan, Ahmed; Min, Kisuk; Zhang, Lei et al. (2018) Skeletal Muscle-Specific Deletion of MKP-1 Reveals a p38 MAPK/JNK/Akt Signaling Node That Regulates Obesity-Induced Insulin Resistance. Diabetes 67:624-635
Vatner, Daniel F; Goedeke, Leigh; Camporez, Joao-Paulo G et al. (2018) Angptl8 antisense oligonucleotide improves adipose lipid metabolism and prevents diet-induced NAFLD and hepatic insulin resistance in rodents. Diabetologia 61:1435-1446
Wang, Yongliang; Nasiri, Ali R; Damsky, William E et al. (2018) Uncoupling Hepatic Oxidative Phosphorylation Reduces Tumor Growth in Two Murine Models of Colon Cancer. Cell Rep 24:47-55

Showing the most recent 10 out of 255 publications