Abstract

The SERCEB Mouse Monoclonal Antibody Core is housed within the Epitope Recogniton and Immunoreagent Core (ERIC) located at the University of Alabama at Birmingham. The core is responsible for the development and large-scale production of mouse Mabs for use by SERCEB members in studies relating to biodefense and emerging infectious diseases. Services provided by the facility include: procurement and housing of pathogen-free rodents, design and implementation of immunization strategies, sera testing, performance effusions and screening assays, specificity testing, cryopreservation of hybridoma lines, subcloning by limiting dilution, isotyping and large-scale production and purification of Mabs. To be of further service to SERCEB investigators, the core is extending its service to include biotyinylation and enzyme conjugation of Mabs. The Mouse Monoclonal Antibody Core has extensive experience in working with offsite investigators. The core has developed flexible protocols that provide investigators with extended periods of time for screening hybridoma supernatants off campus or in BSL3 facilites if necessary.

Public Health Relevance

The monoclonal antibodies produced in this core facility, will play a crucial role in the detection and characterization of infectious agents that are responsible for human disease. Furthermore, some of the antibodies produced in the core, may provide treatment strategies for infectious agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI057157-08
Application #
8036029
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2010-03-01
Budget End
2011-02-28
Support Year
8
Fiscal Year
2010
Total Cost
$192,055
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

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Graham, Rachel L; Deming, Damon J; Deming, Meagan E et al. (2018) Evaluation of a recombination-resistant coronavirus as a broadly applicable, rapidly implementable vaccine platform. Commun Biol 1:179
Qi, Xiaoxuan; Wang, Wenjian; Dong, Haohao et al. (2018) Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus. Methods Mol Biol 1604:179-188
Kocher, Jacob F; Lindesmith, Lisa C; Debbink, Kari et al. (2018) Bat Caliciviruses and Human Noroviruses Are Antigenically Similar and Have Overlapping Histo-Blood Group Antigen Binding Profiles. MBio 9:
Dhanwani, Rekha; Huang, Qinfeng; Lan, Shuiyun et al. (2018) Establishment of Bisegmented and Trisegmented Reverse Genetics Systems to Generate Recombinant Pichindé Viruses. Methods Mol Biol 1604:247-253
Shao, Junjie; Liu, Xiaoying; Liang, Yuying et al. (2018) Assays to Assess Arenaviral Glycoprotein Function. Methods Mol Biol 1604:169-178
Huang, Qinfeng; Shao, Junjie; Liang, Yuying et al. (2018) Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NPs) in Viral RNA Synthesis and in Suppressing Type I Interferon. Methods Mol Biol 1604:189-200
Gunn, Bronwyn M; Jones, Jennifer E; Shabman, Reed S et al. (2018) Ross River virus envelope glycans contribute to disease through activation of the host complement system. Virology 515:250-260
Shao, Junjie; Liang, Yuying; Ly, Hinh (2018) Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interferon-Beta Suppression. Methods Mol Biol 1604:217-227
Wirawan, Melissa; Fibriansah, Guntur; Marzinek, Jan K et al. (2018) Mechanism of Enhanced Immature Dengue Virus Attachment to Endosomal Membrane Induced by prM Antibody. Structure :

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