The ability of arboviruses to persist in novel environments is undoubtedly a function of the rapid adaptability of their RNA genomes, as illustrated by the large number of WNV(18, 72), SLE (71)and JEV (89) genotypic variants that exist in new ecosystems. In addition, considerable genetic data demonstrates a viral genetic component for virulence potential for SLEV (9, 81), WNV (5) and JEV (47, 84) strains. This genetic variability necessitates the identification of conserved viral genetic sequences that can be utilized in the development of genetic tools for viral identification and characterization. In addition to highly specific detection methodologies, new diagnostic assays capable of detecting a wide range of introduced arboviral agents are also needed. Generation of fundamental genetic sequence data on a number of potentially introduced arboviral agents will allow for the targeting of different regions of viral genomes for the development of cross-reactive rapid amplification methodologies that will aid in the improvement of existing surveillance and predictive emergence models for the prevention of human and veterinary disease from introduced viral agents. In human or animal samples where viral genome and antigen may no longer be present, the detection of antibodies can indicate the presence of an introduced agent. Currently, rapid serologic screening assays are non-specific, and the more specific confirmatory assays (e.g., neutralizing antibody assays) require many days of culture and specialized biocontainment laboratories. Rapid (sameday), highly specific serologic assays capable of detecting arboviral reactive irnmunoglobulin, without the need to work with the actual agent, are urgently needed. Under the direction of Dr. Carol Glaser, the VRDL performs standard plaque and other assays for arboviruses of public health importance, such as WNV and SLEV. In the proposed collaborative studies, the VRDL, along with UC Davis investigators, will compare the new molecular assays to standard assays, and will assist in disseminating use of the new assays throughout the California public health system.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54AI065359-03
Application #
7558730
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
3
Fiscal Year
2007
Total Cost
$253,721
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Thongsripong, Panpim; Chandler, James Angus; Green, Amy B et al. (2018) Mosquito vector-associated microbiota: Metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod-borne diseases. Ecol Evol 8:1352-1368
Katzelnick, Leah C; Ben-Shachar, Rotem; Mercado, Juan Carlos et al. (2018) Dynamics and determinants of the force of infection of dengue virus from 1994 to 2015 in Managua, Nicaragua. Proc Natl Acad Sci U S A 115:10762-10767
Clemens, Daniel L; Lee, Bai-Yu; Horwitz, Marcus A (2018) The Francisella Type VI Secretion System. Front Cell Infect Microbiol 8:121
Tsai, Wen-Yang; Youn, Han Ha; Tyson, Jasmine et al. (2018) Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections. Emerg Infect Dis 24:1355-1359
Huwyler, Camille; Heiniger, Nadja; Chomel, Bruno B et al. (2017) Dynamics of Co-Infection with Bartonella henselae Genotypes I and II in Naturally Infected Cats: Implications for Feline Vaccine Development. Microb Ecol 74:474-484
Norris, Michael H; Heacock-Kang, Yun; Zarzycki-Siek, Jan et al. (2017) Burkholderia pseudomallei natural competency and DNA catabolism: Identification and characterization of relevant genes from a constructed fosmid library. PLoS One 12:e0189018
Marques, Adriana R; Yang, Xiuli; Smith, Alexis A et al. (2017) Citrate Anticoagulant Improves the Sensitivity of Borreliella (Borrelia) burgdorferi Plasma Culture. J Clin Microbiol 55:3297-3299
Nualnoi, Teerapat; Norris, Michael H; Tuanyok, Apichai et al. (2017) Development of Immunoassays for Burkholderia pseudomallei Typical and Atypical Lipopolysaccharide Strain Typing. Am J Trop Med Hyg 96:358-367
Parameswaran, Poornima; Wang, Chunling; Trivedi, Surbhi Bharat et al. (2017) Intrahost Selection Pressures Drive Rapid Dengue Virus Microevolution in Acute Human Infections. Cell Host Microbe 22:400-410.e5
Bortell, Nikki; Flynn, Claudia; Conti, Bruno et al. (2017) Osteopontin Impacts West Nile virus Pathogenesis and Resistance by Regulating Inflammasome Components and Cell Death in the Central Nervous System at Early Time Points. Mediators Inflamm 2017:7582437

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