Abstract

Project 6.2. Task-specific Project 2: Multiplexed in-vivo endoscopy by Raman imaging of cancertargeting nanoparticles Principal Investigator: Sam Gambhir Investigators: James Chan, Thomas Huser, Mike Mandella, Sebastian Wachsmann-Hogiu This task-specific project will use nanoparticles (Fig. 20) and intravital Raman imaging at near-infrared wavelengths to image molecular targets in the colon. This project supports aim 1 by providing an alternative set of imaging probes and new instrumentation that may permit multiparametric analysis of Gl cancer. A significant concern for those developing molecular imaging tools is that detection of a single molecular marker may be insufficient for specific detection and staging. Raman imaging of multiple particles with distinct spectra and molecular tags may address this concern. This possibility will be addressed through the following specific aims:
Aim 1 : To develop a compact colonoscope for in-vivo Raman imaging in patients Aim 2: To develop and characterize multiplexed Raman nanoparticles for Gl cancers Aim 3: To image early dysplastic lesions of the colon through in-vivo imaging of Raman nanoprobes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA136465-05
Application #
8379282
Study Section
Special Emphasis Panel (ZCA1-SRRB-9)
Project Start
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
5
Fiscal Year
2012
Total Cost
$48,530
Indirect Cost
$11,547

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Uddin, Md Jashim; Moore, Chauca E; Crews, Brenda C et al. (2016) Fluorocoxib A enables targeted detection of cyclooxygenase-2 in laser-induced choroidal neovascularization. J Biomed Opt 21:90503
Uddin, Md Jashim; Werfel, Thomas A; Crews, Brenda C et al. (2016) Fluorocoxib A loaded nanoparticles enable targeted visualization of cyclooxygenase-2 in inflammation and cancer. Biomaterials 92:71-80
Sensarn, Steven; Zavaleta, Cristina L; Segal, Ehud et al. (2016) A Clinical Wide-Field Fluorescence Endoscopic Device for Molecular Imaging Demonstrating Cathepsin Protease Activity in Colon Cancer. Mol Imaging Biol 18:820-829
Templeton, Zachary S; Bachmann, Michael H; Alluri, Rajiv V et al. (2015) Methods for culturing human femur tissue explants to study breast cancer cell colonization of the metastatic niche. J Vis Exp :
Uddin, Md Jashim; Crews, Brenda C; Ghebreselasie, Kebreab et al. (2015) Targeted imaging of cancer by fluorocoxib C, a near-infrared cyclooxygenase-2 probe. J Biomed Opt 20:50502
Rogalla, Stephan; Contag, Christopher H (2015) Early Cancer Detection at the Epithelial Surface. Cancer J 21:179-87
Garai, Ellis; Sensarn, Steven; Zavaleta, Cristina L et al. (2015) A real-time clinical endoscopic system for intraluminal, multiplexed imaging of surface-enhanced Raman scattering nanoparticles. PLoS One 10:e0123185
Ra, Hyejun; González-González, Emilio; Uddin, Md Jashim et al. (2015) Detection of non-melanoma skin cancer by in vivo fluorescence imaging with fluorocoxib A. Neoplasia 17:201-7
Contag, Christopher H; Lie, Wen-Rong; Bammer, Marie C et al. (2014) Monitoring dynamic interactions between breast cancer cells and human bone tissue in a co-culture model. Mol Imaging Biol 16:158-66
Ajithkumar, G; Yoo, Benjamin; Goral, Dara E et al. (2013) Multimodal bioimaging using rare earth doped Gd2O2S: Yb/Er phosphor with upconversion luminescence and magnetic resonance properties. J Mater Chem A Mater 1:1561-1572

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