TheobjectiveoftheFunctionalGenomicsCore(FGC)istoprovidecuttingedgeandinnovativetechnologies for the functional characterization of the genome in a reliable, reproducible and costefficient manner. It will support?Project1?andProject2byfacilitatingproteomicsexperiments,includingmappingofphysicalprotein interactions,andCRISPRexperiments,includingmappingofgeneticinteractions.Inparticular,theFGCwill perform mass spectrometry characterization, CRISPR gene editing, CRISPRi and CRISPRa screening, and provideexpertiseindataprocessingfortheseexperimentalplatforms.Thiscorewillbecomprisedofthreewell establishedfacilities:theThermoFisherScientificProteomicsFacilityforDiseaseTargetDiscoverylocatedat theJ.DavidGladstoneInstitutes,theUCSDInstituteforGenomicMedicine(IGM)GenomicsCenterandthe UCSDCenterforComputationalBiology&Bioinformatics(CCBB).Additionally,weproposetocreateanew CRISPRscreeningcorethatleveragestheMalilab?s?foundationalexpertiseingenomeengineering. TheFGCcorewillbeledbytwopioneersinthefieldsoffunctionalgenomics,Drs.KroganandMali,whowill employ stateoftheart techniques, many of which were developed or optimized in their respective labs. CoInvestigatorAlanAshworth,aninternationallyrecognizedexpertinbreastcancergenomicsandoneofthe first to demonstrate the therapeutic promise targeting synthetic lethal interactions in cancer, will contribute expertiseintargetselection.Dr.Ashworth?sinputwillensurethatconsiderationsoftranslationalimpactwillbe incorporated at all stages of experimentation. FGC technologies will be used extensively by ?Project 1 ?and Project2?.TheFGCwillnotonlyenableCenterinvestigatorstoaccomplishtheindividualaimsofthisproposal, itwillalsoserveasaresourcetothewiderscientificcommunitybydisseminatingnewassaysandprotocols.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA209891-03
Application #
9692265
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2019-05-01
Budget End
2020-04-30
Support Year
3
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Type
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94118
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