The project will develop a portable microanalytical system based on nanoparticle labels/ immunochromatographic electrochemical biosensor (IEB) for on-site rapid detection and quantification of tobacco smoke exposure (TSE)-induced multiple biomarkers in biological matrices. The detection of TSEinduced biomarkers is based on the principle of immunochromatographic separation and nanoparticleenhanced bioelectrochemical detection. To improve the sensitivity of electrochemical detection in the immunochromatographic platform, apoferritin-templated metallic phosphate nanoparticle labels will be employed to amplify detection signals of those biomarkers. The first goal of this Project is to establish nanoparticle-based competitive electrochemical immunoassay for (a) cotinine as a marker of tobacco smoke exposure. The detection system will be tested and evaluated using human plasma and urine samples (Project 1) and mouse plasma, urine, and saliva (Project 2). Secondly, Project 3 will establish nanoparticlebased multiplexed electrochemical immunoassay for sequence specific nitrotyrosine, sequence - independent nitrotyrosine (identified by Project 1 and 2, and Core A and B) and the pleiotropic, non-specific marker, TNF-alpha. Finally, this Project will build a prototype Immunochromatographic/ Electrochemical Biosensor (IEB) device for on-site application. The device will provide for analysis of exposure (cotinine), selected markers of specific (nitrated proteins) and general markers of stress (e.g. TNF-alpha) in a single platform.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Battelle Pacific Northwest Laboratories
United States
Zip Code
Tanaka, Naoki; Takahashi, Shogo; Hu, Xiao et al. (2017) Growth arrest and DNA damage-inducible 45? protects against nonalcoholic steatohepatitis induced by methionine- and choline-deficient diet. Biochim Biophys Acta Mol Basis Dis 1863:3170-3182
Takahashi, Shogo; Fukami, Tatsuki; Masuo, Yusuke et al. (2016) Cyp2c70 is responsible for the species difference in bile acid metabolism between mice and humans. J Lipid Res 57:2130-2137
Song, Yang; Luo, Yanan; Zhu, Chengzhou et al. (2016) Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials. Biosens Bioelectron 76:195-212
Yang, Guohai; Zhu, Chengzhou; Du, Dan et al. (2015) Graphene-like two-dimensional layered nanomaterials: applications in biosensors and nanomedicine. Nanoscale 7:14217-31
Tanaka, Naoki; Takahashi, Shogo; Zhang, Yuan et al. (2015) Role of fibroblast growth factor 21 in the early stage of NASH induced by methionine- and choline-deficient diet. Biochim Biophys Acta 1852:1242-52
Webb-Robertson, Bobbie-Jo M; Wiberg, Holli K; Matzke, Melissa M et al. (2015) Review, evaluation, and discussion of the challenges of missing value imputation for mass spectrometry-based label-free global proteomics. J Proteome Res 14:1993-2001
Zhu, Chengzhou; Yang, Guohai; Li, He et al. (2015) Electrochemical sensors and biosensors based on nanomaterials and nanostructures. Anal Chem 87:230-49
Tanaka, Naoki; Takahashi, Shogo; Fang, Zhong-Ze et al. (2014) Role of white adipose lipolysis in the development of NASH induced by methionine- and choline-deficient diet. Biochim Biophys Acta 1841:1596-607
Lee, Ai-Cheng; Du, Dan; Chen, Baowei et al. (2014) Electrochemical detection of leukemia oncogenes using enzyme-loaded carbon nanotube labels. Analyst 139:4223-30
Jin, Hongjun; Hallstrand, Teal S; Daly, Don S et al. (2014) A halotyrosine antibody that detects increased protein modifications in asthma patients. J Immunol Methods 403:17-25

Showing the most recent 10 out of 37 publications