2.1. PROJECT 2 (Wagner) ? New Labeling Schemes, NMR Technology, and MP Structure and Function The major objectives of this project are to develop MP reconstitution and isotope labeling methods and new NMR experiments for the proposed pipeline to facilitate MP structure determination by solution NMR. These new developments, in combination with methods to be established in other projects of the Center, will enable routine NMR structure determination of MPs and characterization of MP complexes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM094608-03
Application #
8379222
Study Section
Special Emphasis Panel (ZGM1-CBB-3)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
3
Fiscal Year
2012
Total Cost
$338,294
Indirect Cost
$95,959
Name
Harvard University
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115
Hagn, Franz; Nasr, Mahmoud L; Wagner, Gerhard (2018) Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. Nat Protoc 13:79-98
Min, John; Shih, William M; Bellot, Gaëtan (2017) Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins. Methods Mol Biol 1500:203-215
Kim, Ji-Hun; Schlebach, Jonathan P; Lu, Zhenwei et al. (2016) A pH-Mediated Topological Switch within the N-Terminal Domain of Human Caveolin-3. Biophys J 110:2475-2485
Schlebach, Jonathan P; Barrett, Paul J; Day, Charles A et al. (2016) Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles. Biochemistry 55:985-8
Oxenoid, Kirill; Dong, Ying; Cao, Chan et al. (2016) Architecture of the mitochondrial calcium uniporter. Nature 533:269-73
Dev, Jyoti; Brüschweiler, Sven; Ouyang, Bo et al. (2015) Transverse relaxation dispersion of the p7 membrane channel from hepatitis C virus reveals conformational breathing. J Biomol NMR 61:369-78
Schlebach, Jonathan P; Sanders, Charles R (2015) The safety dance: biophysics of membrane protein folding and misfolding in a cellular context. Q Rev Biophys 48:1-34
Chatterjee, Deep; Eckert, Carl Elias; Slavov, Chavdar et al. (2015) Influence of Arrestin on the Photodecay of Bovine Rhodopsin. Angew Chem Int Ed Engl 54:13555-60
Brüschweiler, Sven; Yang, Qin; Run, Changqing et al. (2015) Substrate-modulated ADP/ATP-transporter dynamics revealed by NMR relaxation dispersion. Nat Struct Mol Biol 22:636-41
Sounier, Remy; Bellot, Gaetan; Chou, James J (2015) Mapping conformational heterogeneity of mitochondrial nucleotide transporter in uninhibited states. Angew Chem Int Ed Engl 54:2436-41

Showing the most recent 10 out of 63 publications