Core 5: Virology, Cell, Molecular and Chemical Biology Core Summary CRNA investigators have a wide range of expertise virology, cell and molecular biology, as well as chemical structural biology. Each of the biologically focused projects requires the application of techniques and expertise in these areas. To facilitate the identification of new targets for structural and biophysical analysis, and to facilitate the validation and biological interpretation of structural and biophysical data, the Virology, Cell, Molecular and Chemical Biology Core will undertake a number of activities including: (1) the discovery of new RNA targets for structure function studies using CLIP (2) the determination of RNA secondary structures in cells using DMS-MaPseq (3) siRNA and sCAS9/SAM screening to identify and validate new protein targets for structure function studies (4) the provision of virology and general molecular biology support to biologically and structurally focused projects and aims (5) virtual screening and chemical synthesis to enable the discovery of RNA- targeted inhibitors of the biological processes studied in the CRNA.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM103297-07
Application #
9557506
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2018-09-01
Budget End
2019-08-31
Support Year
7
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Zhang, Kaiming; Keane, Sarah C; Su, Zhaoming et al. (2018) Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach. Structure 26:490-498.e3
Takata, Matthew A; Soll, Steven J; Emery, Ann et al. (2018) Global synonymous mutagenesis identifies cis-acting RNA elements that regulate HIV-1 splicing and replication. PLoS Pathog 14:e1006824
Bieniasz, Paul D; Kutluay, Sebla B (2018) CLIP-related methodologies and their application to retrovirology. Retrovirology 15:35
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Blanco-Melo, Daniel; Gifford, Robert J; Bieniasz, Paul D (2018) Reconstruction of a replication-competent ancestral murine endogenous retrovirus-L. Retrovirology 15:34
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Shi, Honglue; Clay, Mary C; Rangadurai, Atul et al. (2018) Atomic structures of excited state A-T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations. J Biomol NMR 70:229-244
Kraus, Jodi; Gupta, Rupal; Yehl, Jenna et al. (2018) Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations. J Phys Chem B 122:2931-2939

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