The objectives of this project are to (1) determine if genes present on plasmid DNA of Borrelia burgdorferi, the causative agent of Lyme disease, control infectivity, (2) characterize these plasmids, (3) clone the infectivity genes and express those components that promote borrelia infection in mammals, and (4) determine immunogenic properties of components responsible for infection. The reduction in the number of detectable plasmids with the loss of infectivity suggests that gene(s), encoding for components related to infectivity, may be present on one or more of these extrachromosomal elements. A 7.6 kilobase (kb) pair circular plasmid has been identified as a strong candidate for regulating infection. Various restriction fragments from this plasmid have been cloned and are currently being used as probes for identifying infectious strains. These probes will also be useful in demonstrating the true fate of the 7.6 kb plasmid, which appears to be lost from the spirochete as a result of serial in vitro cultivation. Investigations concerning antigenic variation of B. burgdorferi in vitro and in vivo using a mouse model are continuing. Both monoclonal and monospecific polyclonal antibodies are being developed to help define antigenically variable determinants.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000492-03
Application #
3818266
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Oguge, N O; Durden, L A; Keirans, J E et al. (2009) Ectoparasites (sucking lice, fleas and ticks) of small mammals in southeastern Kenya. Med Vet Entomol 23:387-92
Lopez, Job E; Schrumpf, Merry E; Raffel, Sandra J et al. (2008) Relapsing fever spirochetes retain infectivity after prolonged in vitro cultivation. Vector Borne Zoonotic Dis 8:813-20
Gill, James S; Ullmann, Amy J; Loftis, Amanda D et al. (2008) Novel relapsing fever spirochete in bat tick. Emerg Infect Dis 14:522-3
Mans, Ben J; Andersen, John F; Schwan, Tom G et al. (2008) Characterization of anti-hemostatic factors in the argasid, Argas monolakensis: implications for the evolution of blood-feeding in the soft tick family. Insect Biochem Mol Biol 38:22-41
Battisti, James M; Raffel, Sandra J; Schwan, Tom G (2008) A system for site-specific genetic manipulation of the relapsing fever spirochete Borrelia hermsii. Methods Mol Biol 431:69-84
Dworkin, Mark S; Schwan, Tom G; Anderson Jr, Donald E et al. (2008) Tick-borne relapsing fever. Infect Dis Clin North Am 22:449-68, viii
Mans, Ben J; Andersen, John F; Francischetti, Ivo M B et al. (2008) Comparative sialomics between hard and soft ticks: implications for the evolution of blood-feeding behavior. Insect Biochem Mol Biol 38:42-58
Whitney, Marlyn S; Schwan, Tom G; Sultemeier, Katherine B et al. (2007) Spirochetemia caused by Borrelia turicatae infection in 3 dogs in Texas. Vet Clin Pathol 36:212-6
Pettersson, Jonas; Schrumpf, Merry E; Raffel, Sandra J et al. (2007) Purine salvage pathways among Borrelia species. Infect Immun 75:3877-84
Schwan, Tom G; Raffel, Sandra J; Schrumpf, Merry E et al. (2007) Diversity and distribution of Borrelia hermsii. Emerg Infect Dis 13:436-42

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