We previously reported that human mast cells arise from CD34positive human stem cells in SCF (KIT positive). To further characterize the human mast cell precursor, we sorted CD34positive cells using a variety of candidate markers. The most promising was CD13. A series of experiments based on cell sorting and colony formation then led to the conclusion that CD34 positive, KIT positive, CD13 positive cells gave rise to all mast cells in culture. The regulation of mast cell growth and differentiation depends upon the sequential availability of specific growth factors and the expression of their corresponding receptors. We now have shown that alpha-melanocyte stimulating hormone (alpha-MSH) binds to murine mast cells through its receptor, melanocortin- 1(MC-1). This interaction then increases intracellular CAMP, inhibits IgE-mediated histamine release, and down-modulates mRNAs for IL-1 beta, TNF-alpha and lymphotactin. IL-3 dependent proliferative activity by mast cells was slightly but significantly augmented by alpha-MSH.CD44 is expressed in various isoforms on multiple cell lineages including those of hematopoietic origin and is believed in part to mediate cell adhesion to hyaluronic acid. Using a standard assay, CD34+ derived cultured human mast cells were also demonstrated to adhere to hyaluronic-acid-coated surfaces. Human mast cells were then found by flow cytometry to express CD44S, but not the v5, v6, v7, and v8 isoforms, and to shed CD44S following activation induced by PMA or aggregation of Fcepsilon RI. Thus, human cultured mast cells express and shed CD44S, which appears to mediate the attachment of these cells to hyaluronic acid.
| Bandara, Geethani; Beaven, Michael A; Olivera, Ana et al. (2015) Activated mast cells synthesize and release soluble ST2-a decoy receptor for IL-33. Eur J Immunol 45:3034-44 |
| Kulka, Marianna; Fukuishi, Nobuyuki; Metcalfe, Dean D (2009) Human mast cells synthesize and release angiogenin, a member of the ribonuclease A (RNase A) superfamily. J Leukoc Biol 86:1217-26 |
| Metcalfe, Dean D (2008) Mast cells and mastocytosis. Blood 112:946-56 |
| Soule, Benjamin P; Brown, Jared M; Kushnir-Sukhov, Nataliya M et al. (2007) Effects of gamma radiation on FcepsilonRI and TLR-mediated mast cell activation. J Immunol 179:3276-86 |
| Carter, Melody C; Robyn, Jamie A; Bressler, Peter B et al. (2007) Omalizumab for the treatment of unprovoked anaphylaxis in patients with systemic mastocytosis. J Allergy Clin Immunol 119:1550-1 |
| Swindle, Emily J; Metcalfe, Dean D (2007) The role of reactive oxygen species and nitric oxide in mast cell-dependent inflammatory processes. Immunol Rev 217:186-205 |
| Kushnir-Sukhov, Nataliya M; Brown, Jared M; Wu, Yalin et al. (2007) Human mast cells are capable of serotonin synthesis and release. J Allergy Clin Immunol 119:498-9 |
| Brown, Jared M; Swindle, Emily J; Kushnir-Sukhov, Nataliya M et al. (2007) Silica-directed mast cell activation is enhanced by scavenger receptors. Am J Respir Cell Mol Biol 36:43-52 |
| Sundstrom, J Bruce; Ellis, Jane E; Hair, Gregory A et al. (2007) Human tissue mast cells are an inducible reservoir of persistent HIV infection. Blood 109:5293-300 |
| Prussin, Calman; Metcalfe, Dean D (2006) 5. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 117:S450-6 |
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