The technique of X-ray crystallography, essential to our attempts at structure-assisted vaccine design, is dependent on the relatively poorly uunderstood process of protein crystallization. A central stumbling block in our investigations of the HIV-1 envelope has been the difficulty in obtaining well-diffracting crystals. To solve this problem, we have focused on developing methods to enhance the probability of crystallization.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI005050-02
Application #
6987368
Study Section
(VRC)
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2004
Total Cost
Indirect Cost
Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Huang, Chih-chin; Tang, Min; Zhang, Mei-Yun et al. (2005) Structure of a V3-containing HIV-1 gp120 core. Science 310:1025-8
Majeed, Shahzad; Ofek, Gilad; Belachew, Adam et al. (2003) Enhancing protein crystallization through precipitant synergy. Structure 11:1061-70