The activity of cytosolic phospholipase A2 (cPLA2) may be altered by calcium or by phosphorylation of serines in the cPLA2 molecule. A dual hybridization system in yeast was used to identify protein-protein interactions which might also be involved in the modulation of cPLA2 activity. Using this system, a member of the S-100 family of protein was identified as interacting with cPLA2. Ongoing studies include production of recombinant protein and modulation of enzyme function by oxidant molecules.
Qi, Hai-Yan; Daniels, Mathew P; Liu, Yueqin et al. (2011) A cytosolic phospholipase A2-initiated lipid mediator pathway induces autophagy in macrophages. J Immunol 187:5286-92 |
Qi, Hai-Yan; Shelhamer, James H (2005) Toll-like receptor 4 signaling regulates cytosolic phospholipase A2 activation and lipid generation in lipopolysaccharide-stimulated macrophages. J Biol Chem 280:38969-75 |
Huang, Xiuli; Pawliczak, Rafal; Yao, Xiang-Lan et al. (2003) Characterization of the human p11 promoter sequence. Gene 310:133-42 |
Huang, Xiu-li; Pawliczak, Rafal; Yao, Xiang-lan et al. (2003) Interferon-gamma induces p11 gene and protein expression in human epithelial cells through interferon-gamma-activated sequences in the p11 promoter. J Biol Chem 278:9298-308 |