Abstract

A membrane-regulated, sugar-phosphate: phosphohydrolase has been purified and characterized from Lactobacillus casei. Antibodies to the enzyme have been obtained for use in structure/function experiments. Lactose analogs containing non-metabolizable, (14C)-labeled 2-fluoro-D-glucose or 2-deoxy-D-glucose have been prepared enzymatically. Mutants of Streptococcus lactis have been isolated by positive resistance and UV mutagenesis procedures. These mutants lack ATP-dependent glucokinase and/or PEP-mannose: phosphotransferase activities and are incapable of growth on glucose. Through the use of the bacterial mutants, and transport studies with (14C)-2F-lactose and (14C) 2D-lactose, definitive evidence for intracellular phosphorylation of free sugars via the PTS has been obtained. Biochemical, and physiological studies with Peptostreptococcus anaerobius have revealed that proline is a requirement for the transport and metabolism of glucose by this anaerobic oral pathogen.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000341-04
Application #
4692641
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Zhang, Qiangmin; Gao, Feng; Peng, Hao et al. (2009) Crystal structures of Streptococcus suis mannonate dehydratase (ManD) and its complex with substrate: genetic and biochemical evidence for a catalytic mechanism. J Bacteriol 191:5832-7
Zhang, Qiangmin; Peng, Hao; Gao, Feng et al. (2009) Structural insight into the catalytic mechanism of gluconate 5-dehydrogenase from Streptococcus suis: Crystal structures of the substrate-free and quaternary complex enzymes. Protein Sci 18:294-303
Hall, Barry G; Pikis, Andreas; Thompson, John (2009) Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations. Mol Biol Evol 26:2487-97
Pikis, Andreas; Hess, Sonja; Arnold, Ingrid et al. (2006) Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose. J Biol Chem 281:17900-8
Thompson, John; Hess, Sonja; Pikis, Andreas (2004) Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s). J Biol Chem 279:1553-61
Rajan, Shyamala S; Yang, Xiaojing; Collart, Frank et al. (2004) Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis. Structure 12:1619-29
Yip, Vivian L Y; Varrot, Annabelle; Davies, Gideon J et al. (2004) An unusual mechanism of glycoside hydrolysis involving redox and elimination steps by a family 4 beta-glycosidase from Thermotoga maritima. J Am Chem Soc 126:8354-5
Xu, De-Qi; Thompson, John; Cisar, John O (2003) Genetic loci for coaggregation receptor polysaccharide biosynthesis in Streptococcus gordonii 38. J Bacteriol 185:5419-30
Cisar, J O; Xu, D Q; Thompson, J et al. (2000) An alternative interpretation of nanobacteria-induced biomineralization. Proc Natl Acad Sci U S A 97:11511-5